Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells

Ali Mohammad Tohari, Reem Hasaballah Alhasani, Lincoln Biswas, Sarita Rani Patnaik, James Reilly, Zhihong Zeng, Xinhua Shu

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Abstract

Age-related macular degeneration (AMD), the most common visual disorder in elderly people, is characterized by the formation of deposits beneath the retinal pigment epithelium (RPE) and by dysfunction of RPE and photoreceptor cells. The biologically active form of vitamin D, 1,25-(OH)2D3 (VITD), is categorized as a multifunctional steroid hormone that modulates many transcriptional processes of different genes and is involved in a broad range of cellular functions. Epidemiological and genetic association studies demonstrate that VITD may have a protective role in AMD, while single nucleotide polymorphisms in the vitamin D metabolism gene (CYP24A1) increase the risk of AMD. However, the functional mechanisms of VITD in AMD are not fully understood. In the current study, we investigated the impact of VITD on H2O2-induced oxidative stress and inflammation in human RPE cells. We demonstrate that exposure to H2O2 caused significantly reduced cell viability, increased production of reactive oxygen species (ROS), lowered expression of antioxidant enzymes and enhanced inflammation. VITD exposure notably counteracted the above H2O2-induced effects. Our data suggest that VITD protects the RPE from oxidative damage and elucidate molecular mechanisms of VITD deficiency in the development of AMD.
Original languageEnglish
Article number341
Pages (from-to)341
Journalantioxidants
Volume8
Issue number9
DOIs
Publication statusPublished - 24 Aug 2019

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Retinal Pigments
Macular Degeneration
Vitamin D
Retinal Pigment Epithelium
Epithelial Cells
Inflammation
Photoreceptor Cells
Vertebrate Photoreceptor Cells
Vision Disorders
Genetic Association Studies
Genes
Single Nucleotide Polymorphism
Reactive Oxygen Species
Cell Survival
Oxidative Stress
Antioxidants
Steroids
Hormones
Enzymes

Keywords

  • vitamin D; oxidative stress; inflammation; retinal pigment epithelial cells; age-related macular degeneration

Cite this

Tohari, Ali Mohammad ; Alhasani, Reem Hasaballah ; Biswas, Lincoln ; Patnaik, Sarita Rani ; Reilly, James ; Zeng, Zhihong ; Shu, Xinhua. / Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells. In: antioxidants. 2019 ; Vol. 8, No. 9. pp. 341.
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abstract = "Age-related macular degeneration (AMD), the most common visual disorder in elderly people, is characterized by the formation of deposits beneath the retinal pigment epithelium (RPE) and by dysfunction of RPE and photoreceptor cells. The biologically active form of vitamin D, 1,25-(OH)2D3 (VITD), is categorized as a multifunctional steroid hormone that modulates many transcriptional processes of different genes and is involved in a broad range of cellular functions. Epidemiological and genetic association studies demonstrate that VITD may have a protective role in AMD, while single nucleotide polymorphisms in the vitamin D metabolism gene (CYP24A1) increase the risk of AMD. However, the functional mechanisms of VITD in AMD are not fully understood. In the current study, we investigated the impact of VITD on H2O2-induced oxidative stress and inflammation in human RPE cells. We demonstrate that exposure to H2O2 caused significantly reduced cell viability, increased production of reactive oxygen species (ROS), lowered expression of antioxidant enzymes and enhanced inflammation. VITD exposure notably counteracted the above H2O2-induced effects. Our data suggest that VITD protects the RPE from oxidative damage and elucidate molecular mechanisms of VITD deficiency in the development of AMD.",
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Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells. / Tohari, Ali Mohammad; Alhasani, Reem Hasaballah; Biswas, Lincoln; Patnaik, Sarita Rani ; Reilly, James; Zeng, Zhihong; Shu, Xinhua.

In: antioxidants, Vol. 8, No. 9, 341, 24.08.2019, p. 341.

Research output: Contribution to journalArticle

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AU - Alhasani, Reem Hasaballah

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AU - Zeng, Zhihong

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