Viability of Saccharomyces cerevisiae incorporated within silica and polysaccharide hosts monitored via time-resolved fluorescence

A. Sheila Holmes-Smith, Alexis C. Hollas, David McLoskey, Graham Hungerford

    Research output: Contribution to journalArticle

    Abstract

    The viability of Saccharomyces cerevisiae in biocompatible polymers under different growth conditions and studied using time-resolved fluorescence techniques is presented. Two fluorophores, the viscosity sensitive probe 4-(4-(dimethylamino)styryl)-N-methyl-pyridiniumiodine (DASPMI) and the yeast viability stain 2-chloro-4-(2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)-methy lidenr)-1-phenylquinolinium iodide (FUN-1) are used to elucidate information on the incorporated yeast cell viability. Variations in cell viscosity, which are indicative of the cell state, were obtained using DASPMI. Prior to observing FUN-1 in yeast cells using fluorescence lifetime imaging, its photophysics in solution and heterogeneous media were investigated. Time-resolved emission spectra were measured and analysed to associate lifetimes to the spectral emission. Preliminary results show that monitoring the fluorescence lifetime of FUN-1 may give a useful insight into cellular metabolism.
    Original languageEnglish
    Pages (from-to)2186-2194
    Number of pages9
    Journal Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences
    Volume12
    Early online date21 Oct 2013
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Saccharomyces cerevisiae
    • fluorescence techniques
    • cellular metabolism

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