Abstract
The viability of Saccharomyces cerevisiae in biocompatible polymers under different growth conditions and studied using time-resolved fluorescence techniques is presented. Two fluorophores, the viscosity sensitive probe 4-(4-(dimethylamino)styryl)-N-methyl-pyridiniumiodine (DASPMI) and the yeast viability stain 2-chloro-4-(2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)-methy lidenr)-1-phenylquinolinium iodide (FUN-1) are used to elucidate information on the incorporated yeast cell viability. Variations in cell viscosity, which are indicative of the cell state, were obtained using DASPMI. Prior to observing FUN-1 in yeast cells using fluorescence lifetime imaging, its photophysics in solution and heterogeneous media were investigated. Time-resolved emission spectra were measured and analysed to associate lifetimes to the spectral emission. Preliminary results show that monitoring the fluorescence lifetime of FUN-1 may give a useful insight into cellular metabolism.
Original language | English |
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Pages (from-to) | 2186-2194 |
Number of pages | 9 |
Journal | Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences |
Volume | 12 |
Early online date | 21 Oct 2013 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- Saccharomyces cerevisiae
- fluorescence techniques
- cellular metabolism