Vaccination against clostridium difficile using toxin fragments: observations and analysis in animal models.

Janice Spencer, Rosanna Leuzzi, Anthony Buckley, June Irvine, Denise Candlish, Maria Scarselli, Gillian R. Douce*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Clostridium difficile is a major cause of antibiotic associated diarrhea. Recently, we have shown that effective protection can be mediated in hamsters through the inclusion of specific recombinant fragments from toxin A and B in a systemically delivered vaccine. Interestingly while neutralizing antibodies to the binding domains of both toxin A and B are moderately protective, enhanced survival is observed when fragments from the glucosyltransferase region of toxin B replace those from the binding domain of this toxin. In this addendum, we discuss additional information that has been derived from such vaccination studies. This includes observations on efficacy and cross-protection against different ribotypes mediated by these vaccines and the challenges that remain for a vaccine which prevents clinical symptoms but not colonization. The use and value of vaccination both in the prevention of infection and for treatment of disease relapse will be discussed.
Original languageEnglish
Pages (from-to)225-232
Number of pages8
JournalGut Microbes
Volume5
Issue number2
DOIs
Publication statusPublished - 22 Jan 2014

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Keywords

  • clostridium difficile
  • vaccination
  • toxin fragments
  • neutralizing antibodies
  • hamster models
  • diarrhea
  • colonization factors
  • glucosyltransferase activity
  • protection

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