Use of lanthanide-containing polyoxometalates to sensitise the emission of fluorescent labelled serum albumin

A. Sheila Holmes-Smith, Jacob Crisp, Firasat Hussain, Greta R. Patzke, Graham Hungerford*

*Corresponding author for this work

    Research output: Contribution to journalArticle

    8 Downloads (Pure)

    Abstract

    Monitoring the interaction of biomolecules is important, and the use of energy transfer is a principal technique in elucidating nanoscale interactions. Lanthanide compounds are promising luminescent probes for biological samples as their emission is longer-lived than any native autofluorescence. Polyoxometalates (POMs) are interesting structural motifs to incorporate lanthanides, offering low toxicity and a size pertinent for biological applications. Here, we employ iso-structured POMs containing either terbium or europium and assess their interaction with serum albumin by sensitisation of a fluorescent tag on the protein via LRET (luminescence resonance energy transfer) by exciting the lanthanide. Time-resolved measurements showed energy transfer with an efficiency of over 90% for the POM–protein systems. The Tb–POM results were relatively straightforward, while those with the iso-structured Eu–POM were complicated by the effect of protein shielding from the aqueous environment.
    Original languageEnglish
    Pages (from-to)418–424
    Number of pages7
    JournalChemPhysChem : a European Journal of Chemical Physics and Physical Chemistry
    Volume17
    Issue number3
    Early online date7 Dec 2015
    DOIs
    Publication statusPublished - 3 Feb 2016

    Keywords

    • europium
    • LRET
    • protein
    • terbiuim
    • time-resolved luminescence

    Fingerprint Dive into the research topics of 'Use of lanthanide-containing polyoxometalates to sensitise the emission of fluorescent labelled serum albumin'. Together they form a unique fingerprint.

  • Profiles

    Cite this