Abstract
The use of mupirocin (Bactroban ointment) in the Scottish MRSA screening policy requires further investigation as not only has effectiveness been questioned and the possibility of increased resistance levels been identified, but there is the additional likelihood of the full course of treatment not being completed.
Current antibiotic sensitivity testing methods often fail to evaluate susceptibility of biofilm-associated cells, expose cells to a single dose of antimicrobial or employ static culture conditions unrealistic of the clinical situation. This study aimed to develop a model which could be used to evaluate eradication of MRSA biofilms under conditions representative of current treatment recommendations. MRSA biofilms were cultivated under flow conditions and exposed at fixed time intervals to clinically relevant concentrations of mupirocin to reflect the peaks and troughs encountered during therapy, generating a model for the further investigation of how the impact of mupirocin can be maximised for the nasal decolonisation of patients
Current antibiotic sensitivity testing methods often fail to evaluate susceptibility of biofilm-associated cells, expose cells to a single dose of antimicrobial or employ static culture conditions unrealistic of the clinical situation. This study aimed to develop a model which could be used to evaluate eradication of MRSA biofilms under conditions representative of current treatment recommendations. MRSA biofilms were cultivated under flow conditions and exposed at fixed time intervals to clinically relevant concentrations of mupirocin to reflect the peaks and troughs encountered during therapy, generating a model for the further investigation of how the impact of mupirocin can be maximised for the nasal decolonisation of patients
| Original language | English |
|---|---|
| Publisher | Scottish Infection Research Network |
| Publication status | Unpublished - 2013 |
Keywords
- in vitro model
- nasal colonisation
- MRSA
- mupirocin decolonisation