To develop an in vitro model of nasal colonisation by MRSA to investigate the efficiency of mupirocin decolonisation

Susan Lang, Andrew Anyakwo, Lesley Price

Research output: Book/ReportOther report

Abstract

The use of mupirocin (Bactroban ointment) in the Scottish MRSA screening policy requires further investigation as not only has effectiveness been questioned and the possibility of increased resistance levels been identified, but there is the additional likelihood of the full course of treatment not being completed.

Current antibiotic sensitivity testing methods often fail to evaluate susceptibility of biofilm-associated cells, expose cells to a single dose of antimicrobial or employ static culture conditions unrealistic of the clinical situation. This study aimed to develop a model which could be used to evaluate eradication of MRSA biofilms under conditions representative of current treatment recommendations. MRSA biofilms were cultivated under flow conditions and exposed at fixed time intervals to clinically relevant concentrations of mupirocin to reflect the peaks and troughs encountered during therapy, generating a model for the further investigation of how the impact of mupirocin can be maximised for the nasal decolonisation of patients
Original languageEnglish
Publisher Scottish Infection Research Network
Publication statusUnpublished - 2013

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Mupirocin
Methicillin-Resistant Staphylococcus aureus
Nose
Biofilms
Ointments
Therapeutics
Anti-Bacterial Agents
In Vitro Techniques

Keywords

  • in vitro model
  • nasal colonisation
  • MRSA
  • mupirocin decolonisation

Cite this

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To develop an in vitro model of nasal colonisation by MRSA to investigate the efficiency of mupirocin decolonisation. / Lang, Susan; Anyakwo, Andrew; Price, Lesley.

Scottish Infection Research Network, 2013.

Research output: Book/ReportOther report

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AU - Anyakwo, Andrew

AU - Price, Lesley

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AB - The use of mupirocin (Bactroban ointment) in the Scottish MRSA screening policy requires further investigation as not only has effectiveness been questioned and the possibility of increased resistance levels been identified, but there is the additional likelihood of the full course of treatment not being completed.Current antibiotic sensitivity testing methods often fail to evaluate susceptibility of biofilm-associated cells, expose cells to a single dose of antimicrobial or employ static culture conditions unrealistic of the clinical situation. This study aimed to develop a model which could be used to evaluate eradication of MRSA biofilms under conditions representative of current treatment recommendations. MRSA biofilms were cultivated under flow conditions and exposed at fixed time intervals to clinically relevant concentrations of mupirocin to reflect the peaks and troughs encountered during therapy, generating a model for the further investigation of how the impact of mupirocin can be maximised for the nasal decolonisation of patients

KW - in vitro model

KW - nasal colonisation

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BT - To develop an in vitro model of nasal colonisation by MRSA to investigate the efficiency of mupirocin decolonisation

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