The effect of surface treatment of silicone hydrogel contact lenses on the attachment of Acanthamoeba castellanii trophozoites

Tara K. Beattie; Alan Tomlinson

doi:10.1097/ICL.0b013e3181becce6

The effect of surface treatment of silicone hydrogel contact lenses on the attachment of Acanthamoeba castellanii trophozoites

Tara K. Beattie, Alan Tomlinson

Vision Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

This study aimed to determine if plasma surface treatment of Focus Night & Day silicone hydrogel contact lenses affects the attachment of Acanthamoeba. Unworn lotrafilcon A contact lenses with (Focus Night & Day) and without surface treatment and Acuvue, conventional hydrogel lenses, were quartered before 90-min incubation with Acanthamoeba castellanii trophozoites. After incubation and rinsing, the trophozoites attached to one surface of each quarter were counted by direct light microscopy. Sixteen replicates were observed for each lens type. Logarithmic transformation of data allowed the use of parametric analysis of variance.

Original language	English
Pages (from-to)	316-319
Number of pages	4
Journal	Eye and Contact Lens: Science and Clinical Practice
Volume	35
Issue number	6
DOIs	https://doi.org/10.1097/ICL.0b013e3181becce6
Publication status	Published - 1 Nov 2009

Keywords

contact lenses
trophozoites
vision sciences

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10.1097/ICL.0b013e3181becce6

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title = "The effect of surface treatment of silicone hydrogel contact lenses on the attachment of Acanthamoeba castellanii trophozoites",

abstract = "This study aimed to determine if plasma surface treatment of Focus Night & Day silicone hydrogel contact lenses affects the attachment of Acanthamoeba. Unworn lotrafilcon A contact lenses with (Focus Night & Day) and without surface treatment and Acuvue, conventional hydrogel lenses, were quartered before 90-min incubation with Acanthamoeba castellanii trophozoites. After incubation and rinsing, the trophozoites attached to one surface of each quarter were counted by direct light microscopy. Sixteen replicates were observed for each lens type. Logarithmic transformation of data allowed the use of parametric analysis of variance.",

keywords = "contact lenses, trophozoites, vision sciences",

author = "Beattie, {Tara K.} and Alan Tomlinson",

note = "<p>Originally published in: Eye and Contact Lens: Science and Clinical Practice (2009), 35 (6), pp.316-319.</p>",

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AU - Beattie, Tara K.

AU - Tomlinson, Alan

N1 - <p>Originally published in: Eye and Contact Lens: Science and Clinical Practice (2009), 35 (6), pp.316-319.</p>

PY - 2009/11/1

Y1 - 2009/11/1

N2 - This study aimed to determine if plasma surface treatment of Focus Night & Day silicone hydrogel contact lenses affects the attachment of Acanthamoeba. Unworn lotrafilcon A contact lenses with (Focus Night & Day) and without surface treatment and Acuvue, conventional hydrogel lenses, were quartered before 90-min incubation with Acanthamoeba castellanii trophozoites. After incubation and rinsing, the trophozoites attached to one surface of each quarter were counted by direct light microscopy. Sixteen replicates were observed for each lens type. Logarithmic transformation of data allowed the use of parametric analysis of variance.

AB - This study aimed to determine if plasma surface treatment of Focus Night & Day silicone hydrogel contact lenses affects the attachment of Acanthamoeba. Unworn lotrafilcon A contact lenses with (Focus Night & Day) and without surface treatment and Acuvue, conventional hydrogel lenses, were quartered before 90-min incubation with Acanthamoeba castellanii trophozoites. After incubation and rinsing, the trophozoites attached to one surface of each quarter were counted by direct light microscopy. Sixteen replicates were observed for each lens type. Logarithmic transformation of data allowed the use of parametric analysis of variance.

KW - contact lenses

KW - trophozoites

KW - vision sciences

U2 - 10.1097/ICL.0b013e3181becce6

DO - 10.1097/ICL.0b013e3181becce6

M3 - Article

SN - 1542-233X)

VL - 35

SP - 316

EP - 319

JO - Eye and Contact Lens: Science and Clinical Practice

JF - Eye and Contact Lens: Science and Clinical Practice

IS - 6

ER -

The effect of surface treatment of silicone hydrogel contact lenses on the attachment of Acanthamoeba castellanii trophozoites

Abstract

Keywords

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