TY - JOUR
T1 - The effect of chitosan incorporation on physico-mechanical and biological characteristics of a calcium silicate filling material
AU - Abusrewil, Sumaya
AU - Scott, J. Alun
AU - Alqahtari, Saeed S.
AU - Butcher, Mark C.
AU - Tiba, Mohammed
AU - Kumar, Charchit
AU - Mulvihill, Daniel M.
AU - Ramage, Gordon
AU - McLean, William
PY - 2024/4
Y1 - 2024/4
N2 - Objectives: A tricalcium silicate-based cement, Biodentine™, has displayed antibiofilm activity when mixed with chitosan powder. This study aimed to assess the effect of chitosan incorporation on the physico-mechanical and biological properties of Biodentine™. Methods: In this study, me-dium molecular weight chitosan powder was incorporated into Biodentine™ in varying propor-tions (2.5 wt%, 5 wt%, 10 wt%, and 20 wt%). The setting time was determined using a Vicat appa-ratus, solubility was assessed by calculating weight variation after water immersion, radiopacity was evaluated and expressed in millimeters of aluminum, the compressive strength was evaluated using an Instron testing machine, and the microhardness was measured with a Vickers micro-hardness tester. In addition, surface topography of specimens was analyzed using scanning elec-tron microscopy, and the effect of chitosan on the viability of human embryonic kidney (HEK 293) cells was measured by a colorimetric MTT assay. Results: Incorporation of 2.5 wt% and 5 wt% chi-tosan powder delivered an advantage by speeding up the setting time of Biodentine material. However, the incorporation of chitosan compromised all other material properties and the crys-talline structure in a dose-dependent manner. The chitosan-modified material also showed signifi-cant decreases in the proliferation of the HEK 293 cells, signifying decreased biocompatibility. Sig-nificance: Chitosan incorporation into calcium silicate materials adversely affects the physical and biological properties of the material. Despite the increased antimicrobial activity of the modified material, the diminution in these properties is likely to reduce its clinical value.
AB - Objectives: A tricalcium silicate-based cement, Biodentine™, has displayed antibiofilm activity when mixed with chitosan powder. This study aimed to assess the effect of chitosan incorporation on the physico-mechanical and biological properties of Biodentine™. Methods: In this study, me-dium molecular weight chitosan powder was incorporated into Biodentine™ in varying propor-tions (2.5 wt%, 5 wt%, 10 wt%, and 20 wt%). The setting time was determined using a Vicat appa-ratus, solubility was assessed by calculating weight variation after water immersion, radiopacity was evaluated and expressed in millimeters of aluminum, the compressive strength was evaluated using an Instron testing machine, and the microhardness was measured with a Vickers micro-hardness tester. In addition, surface topography of specimens was analyzed using scanning elec-tron microscopy, and the effect of chitosan on the viability of human embryonic kidney (HEK 293) cells was measured by a colorimetric MTT assay. Results: Incorporation of 2.5 wt% and 5 wt% chi-tosan powder delivered an advantage by speeding up the setting time of Biodentine material. However, the incorporation of chitosan compromised all other material properties and the crys-talline structure in a dose-dependent manner. The chitosan-modified material also showed signifi-cant decreases in the proliferation of the HEK 293 cells, signifying decreased biocompatibility. Sig-nificance: Chitosan incorporation into calcium silicate materials adversely affects the physical and biological properties of the material. Despite the increased antimicrobial activity of the modified material, the diminution in these properties is likely to reduce its clinical value.
U2 - 10.3390/dj12040100
DO - 10.3390/dj12040100
M3 - Article
SN - 2304-6767
VL - 12
JO - Dentistry Journal
JF - Dentistry Journal
IS - 4
M1 - 100
ER -