The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function

Jennifer A. Easton; Jorgen S. Petersen; Patricia E.M. Martin

doi:10.1007/s00210-009-0411-2

The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function

Jennifer A. Easton, Jorgen S. Petersen, Patricia E.M. Martin

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.

Original language	English
Pages (from-to)	11-24
Number of pages	14
Journal	Naunyn-Schmiedeberg's Archives of Pharmacology
Volume	380
Issue number	1
DOIs	https://doi.org/10.1007/s00210-009-0411-2
Publication status	Published - 1 Mar 2009

Keywords

connexin
gap junction
anti-arrhythmic peptide
intercellular communication

Documents and Links

10.1007/s00210-009-0411-2

http://researchonline.gcu.ac.uk/sls/292

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title = "The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function",

abstract = "The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.",

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author = "Easton, {Jennifer A.} and Petersen, {Jorgen S.} and Martin, {Patricia E.M.}",

note = "Originally published in: Naunyn-Schmiedebergs Archives of Pharmacology (2009), 380 (1), pp.11-24.",

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T1 - The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function

AU - Easton, Jennifer A.

AU - Petersen, Jorgen S.

AU - Martin, Patricia E.M.

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N2 - The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.

AB - The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.

KW - connexin

KW - gap junction

KW - anti-arrhythmic peptide

KW - intercellular communication

U2 - 10.1007/s00210-009-0411-2

DO - 10.1007/s00210-009-0411-2

M3 - Article

VL - 380

SP - 11

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JO - Naunyn-Schmiedeberg's Archives of Pharmacology

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