Abstract
The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 11-24 |
| Number of pages | 14 |
| Journal | Naunyn-Schmiedebergs Archives of Pharmacology |
| Volume | 380 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Mar 2009 |
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Keywords
- connexin
- gap junction
- anti-arrhythmic peptide
- intercellular communication
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The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function. / Easton, Jennifer A.; Petersen, Jorgen S.; Martin, Patricia E.M.
In: Naunyn-Schmiedebergs Archives of Pharmacology, Vol. 380, No. 1, 01.03.2009, p. 11-24.Research output: Contribution to journal › Article
TY - JOUR
T1 - The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function
AU - Easton, Jennifer A.
AU - Petersen, Jorgen S.
AU - Martin, Patricia E.M.
N1 - Originally published in: Naunyn-Schmiedebergs Archives of Pharmacology (2009), 380 (1), pp.11-24.
PY - 2009/3/1
Y1 - 2009/3/1
N2 - The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.
AB - The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.
KW - connexin
KW - gap junction
KW - anti-arrhythmic peptide
KW - intercellular communication
U2 - 10.1007/s00210-009-0411-2
DO - 10.1007/s00210-009-0411-2
M3 - Article
VL - 380
SP - 11
EP - 24
IS - 1
ER -