The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function

Jennifer A. Easton, Jorgen S. Petersen, Patricia E.M. Martin

Research output: Contribution to journalArticle

Abstract

The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.

Original languageEnglish
Pages (from-to)11-24
Number of pages14
JournalNaunyn-Schmiedebergs Archives of Pharmacology
Volume380
Issue number1
DOIs
Publication statusPublished - 1 Mar 2009

Fingerprint

Connexin 43
Anti-Arrhythmia Agents
Gap Junctions
Connexins
Protein C Inhibitor
Microinjections
Protein Kinase Inhibitors
Fluorescent Dyes
HeLa Cells
Protein Kinase C
Up-Regulation
Peptides
AAP 10

Keywords

  • connexin
  • gap junction
  • anti-arrhythmic peptide
  • intercellular communication

Cite this

Easton, J. A., Petersen, J. S., & Martin, P. E. M. (2009). The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function. Naunyn-Schmiedebergs Archives of Pharmacology, 380(1), 11-24. https://doi.org/10.1007/s00210-009-0411-2
Easton, Jennifer A. ; Petersen, Jorgen S. ; Martin, Patricia E.M. / The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function. In: Naunyn-Schmiedebergs Archives of Pharmacology. 2009 ; Vol. 380, No. 1. pp. 11-24.
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Easton, JA, Petersen, JS & Martin, PEM 2009, 'The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function', Naunyn-Schmiedebergs Archives of Pharmacology, vol. 380, no. 1, pp. 11-24. https://doi.org/10.1007/s00210-009-0411-2

The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function. / Easton, Jennifer A.; Petersen, Jorgen S.; Martin, Patricia E.M.

In: Naunyn-Schmiedebergs Archives of Pharmacology, Vol. 380, No. 1, 01.03.2009, p. 11-24.

Research output: Contribution to journalArticle

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T1 - The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function

AU - Easton, Jennifer A.

AU - Petersen, Jorgen S.

AU - Martin, Patricia E.M.

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AB - The anti-arrhythmic peptide AAP10 has previously been shown to acutely upregulate electrical cell-to-cell coupling mediated via connexin 43 gap junctions. In the present work, we have further examined the connexin (Cx) specificity and mechanism of action of this peptide in HeLa cells expressing Cx43, Cx40 or Cx26. The ability of cells to transfer the small fluorescent dyes Alexa 488 (MW 570) or Alexa 594 (MW 759), as markers for metabolic coupling mediated via gap junctions, before and after exposure to AAP10 and/or the protein kinase C inhibitor chelerythrine for 5 h was determined by microinjection analysis.

KW - connexin

KW - gap junction

KW - anti-arrhythmic peptide

KW - intercellular communication

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Easton JA, Petersen JS, Martin PEM. The anti-arrhythmic peptide AAP10 remodels Cx43 and Cx40 expression and function. Naunyn-Schmiedebergs Archives of Pharmacology. 2009 Mar 1;380(1):11-24. https://doi.org/10.1007/s00210-009-0411-2