Abstract
A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).
| Original language | English |
|---|---|
| Pages (from-to) | 639-646 |
| Number of pages | 8 |
| Journal | Marine Biology |
| Volume | 157 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Jan 2010 |
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Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis). / Sedik, Wael F.; Dempsey, Kate E.; Meng, Xiang; Craft, John A.
In: Marine Biology, Vol. 157, No. 3, 01.01.2010, p. 639-646.Research output: Contribution to journal › Article
TY - JOUR
T1 - Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis)
AU - Sedik, Wael F.
AU - Dempsey, Kate E.
AU - Meng, Xiang
AU - Craft, John A.
N1 - Originally published in: Marine Biology (2010), 157 (3), pp.639-646.
PY - 2010/1/1
Y1 - 2010/1/1
N2 - A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).
AB - A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).
U2 - 10.1007/s00227-009-1349-0
DO - 10.1007/s00227-009-1349-0
M3 - Article
VL - 157
SP - 639
EP - 646
JO - Marine Biology
JF - Marine Biology
SN - 0025-3162
IS - 3
ER -