Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis)

Wael F. Sedik; Kate E. Dempsey; Xiang Meng; John A. Craft

doi:10.1007/s00227-009-1349-0

Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis)

Wael F. Sedik, Kate E. Dempsey, Xiang Meng, John A. Craft

Biological and Biomedical Sciences

Research output: Contribution to journal › Article

Abstract

A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).

Original language	English
Pages (from-to)	639-646
Number of pages	8
Journal	Marine Biology
Volume	157
Issue number	3
DOIs	https://doi.org/10.1007/s00227-009-1349-0
Publication status	Published - 1 Jan 2010

Documents and Links

10.1007/s00227-009-1349-0

http://researchonline.gcu.ac.uk/sls/240

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Sedik, W. F., Dempsey, K. E., Meng, X., & Craft, J. A. (2010). Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis). Marine Biology, 157(3), 639-646. https://doi.org/10.1007/s00227-009-1349-0

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abstract = "A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).",

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AU - Sedik, Wael F.

AU - Dempsey, Kate E.

AU - Meng, Xiang

AU - Craft, John A.

N1 - Originally published in: Marine Biology (2010), 157 (3), pp.639-646.

PY - 2010/1/1

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N2 - A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).

AB - A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).

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DO - 10.1007/s00227-009-1349-0

M3 - Article

VL - 157

SP - 639

EP - 646

JO - Marine Biology

JF - Marine Biology

SN - 0025-3162

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