Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis)

Wael F. Sedik, Kate E. Dempsey, Xiang Meng, John A. Craft

Research output: Contribution to journalArticle

Abstract

A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).

Original languageEnglish
Pages (from-to)639-646
Number of pages8
JournalMarine Biology
Volume157
Issue number3
DOIs
Publication statusPublished - 1 Jan 2010

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histology
Mytilus edulis
mussels
reverse transcriptase polymerase chain reaction
assay
mantle
receptors
gene
gender
assays
genes
methodology
method

Cite this

Sedik, Wael F. ; Dempsey, Kate E. ; Meng, Xiang ; Craft, John A. / Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis). In: Marine Biology. 2010 ; Vol. 157, No. 3. pp. 639-646.
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Temporal expression of sex-specific genes in the mantle of the common mussel (Mytilus edulis). / Sedik, Wael F.; Dempsey, Kate E.; Meng, Xiang; Craft, John A.

In: Marine Biology, Vol. 157, No. 3, 01.01.2010, p. 639-646.

Research output: Contribution to journalArticle

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AU - Sedik, Wael F.

AU - Dempsey, Kate E.

AU - Meng, Xiang

AU - Craft, John A.

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AB - A qPCR assay was developed to measure expression of male-specific vitelline coat lysin (VCL) and female-specific vitelline envelop receptor for lysin (VERL) in the mantle of Mytilus edulis, the common mussel. The ability of the method to correctly assign sex was validated by a combination of histology and a previously developed end-point RT–PCR for these transcripts (Hines et al. 2007).

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EP - 646

JO - Marine Biology

JF - Marine Biology

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