Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR

Margaret Brown; Craig Robinson; Ian M. Davies; Colin F. Moffat; John Redshaw; John A. Craft

doi:10.1016/j.mrfmmm.2004.06.002

Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR

Margaret Brown, Craig Robinson, Ian M. Davies, Colin F. Moffat, John Redshaw, John A. Craft

Biological and Biomedical Sciences

Research output: Contribution to journal › Article

Abstract

Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially-expressed in liver from male plaice (Pleuronectes platessa) exposed to ethynyl oestradiol (EE2). BLAST analysis and alignments of the clones with database sequence suggested at least three vitellogenin (VTG) genes and three zona radiata protein (ZRP) genes were represented. Clones with unique sequence (62 up-, 13 down-regulated) were arrayed as probes on nylon membranes to investigate temporal expression of oestrogen-responsive genes in experimental animals. Arrays were hybridised with radiolabelled cDNAs prepared from hepatic mRNA from animals treated with EE2 for various times upto 21 days and from treated animals transferred to clean water for upto a further 31 days.

Original language	English
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
DOIs	https://doi.org/10.1016/j.mrfmmm.2004.06.002
Publication status	Published - 1 Aug 2004

Keywords

Pleuronectes platessa
Vitellogenin
ethynyl oestradiol
endocrine disruption
macroarray

Documents and Links

10.1016/j.mrfmmm.2004.06.002

http://researchonline.gcu.ac.uk/sls/26

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Brown, M., Robinson, C., Davies, I. M., Moffat, C. F., Redshaw, J., & Craft, J. A. (2004). Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. https://doi.org/10.1016/j.mrfmmm.2004.06.002

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title = "Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR",

abstract = "Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially-expressed in liver from male plaice (Pleuronectes platessa) exposed to ethynyl oestradiol (EE2). BLAST analysis and alignments of the clones with database sequence suggested at least three vitellogenin (VTG) genes and three zona radiata protein (ZRP) genes were represented. Clones with unique sequence (62 up-, 13 down-regulated) were arrayed as probes on nylon membranes to investigate temporal expression of oestrogen-responsive genes in experimental animals. Arrays were hybridised with radiolabelled cDNAs prepared from hepatic mRNA from animals treated with EE2 for various times upto 21 days and from treated animals transferred to clean water for upto a further 31 days.",

keywords = "Pleuronectes platessa, Vitellogenin, ethynyl oestradiol, endocrine disruption, macroarray",

author = "Margaret Brown and Craig Robinson and Davies, {Ian M.} and Moffat, {Colin F.} and John Redshaw and Craft, {John A.}",

note = "<p>Originally published in: Mutation Research (2004), 552 (1-2), pp.35-49.</p>",

year = "2004",

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doi = "10.1016/j.mrfmmm.2004.06.002",

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Brown, M, Robinson, C, Davies, IM, Moffat, CF, Redshaw, J & Craft, JA 2004, 'Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. https://doi.org/10.1016/j.mrfmmm.2004.06.002

Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR. / Brown, Margaret; Robinson, Craig; Davies, Ian M.; Moffat, Colin F.; Redshaw, John; Craft, John A.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 01.08.2004.

Research output: Contribution to journal › Article

TY - JOUR

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AU - Brown, Margaret

AU - Robinson, Craig

AU - Davies, Ian M.

AU - Moffat, Colin F.

AU - Redshaw, John

AU - Craft, John A.

N1 - <p>Originally published in: Mutation Research (2004), 552 (1-2), pp.35-49.</p>

PY - 2004/8/1

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AB - Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially-expressed in liver from male plaice (Pleuronectes platessa) exposed to ethynyl oestradiol (EE2). BLAST analysis and alignments of the clones with database sequence suggested at least three vitellogenin (VTG) genes and three zona radiata protein (ZRP) genes were represented. Clones with unique sequence (62 up-, 13 down-regulated) were arrayed as probes on nylon membranes to investigate temporal expression of oestrogen-responsive genes in experimental animals. Arrays were hybridised with radiolabelled cDNAs prepared from hepatic mRNA from animals treated with EE2 for various times upto 21 days and from treated animals transferred to clean water for upto a further 31 days.

KW - Pleuronectes platessa

KW - Vitellogenin

KW - ethynyl oestradiol

KW - endocrine disruption

KW - macroarray

U2 - 10.1016/j.mrfmmm.2004.06.002

DO - 10.1016/j.mrfmmm.2004.06.002

M3 - Article

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

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SN - 0027-5107

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