Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR

Margaret Brown, Craig Robinson, Ian M. Davies, Colin F. Moffat, John Redshaw, John A. Craft

Research output: Contribution to journalArticle

Abstract

Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially-expressed in liver from male plaice (Pleuronectes platessa) exposed to ethynyl oestradiol (EE2). BLAST analysis and alignments of the clones with database sequence suggested at least three vitellogenin (VTG) genes and three zona radiata protein (ZRP) genes were represented. Clones with unique sequence (62 up-, 13 down-regulated) were arrayed as probes on nylon membranes to investigate temporal expression of oestrogen-responsive genes in experimental animals. Arrays were hybridised with radiolabelled cDNAs prepared from hepatic mRNA from animals treated with EE2 for various times upto 21 days and from treated animals transferred to clean water for upto a further 31 days.

Keywords

  • Pleuronectes platessa
  • Vitellogenin
  • ethynyl oestradiol
  • endocrine disruption
  • macroarray

Fingerprint Dive into the research topics of 'Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and real-time PCR'. Together they form a unique fingerprint.

  • Cite this