Targeting mitochondrial 18 kDa translocator protein (TSPO) regulates macrophage cholesterol efflux and lipid phenotype

Janice Taylor, Anne Marie Allen, Ann Graham

Research output: Contribution to journalArticle

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Abstract

The aim of the present study was to establish mitochondrial cholesterol trafficking 18 kDa translocator protein (TSPO) as a potential therapeutic target, capable of increasing macrophage cholesterol efflux to (apo)lipoprotein acceptors. Expression and activity of TSPO in human (THP-1) macrophages were manipulated genetically and by the use of selective TSPO ligands. Cellular responses were analysed by quantitative PCR (Q-PCR), immunoblotting and radiolabelling, including [3H]cholesterol efflux to (apo)lipoprotein A-I (apoA-I), high-density lipoprotein (HDL) and human serum. Induction of macrophage cholesterol deposition by acetylated low-density lipoprotein (AcLDL) increased expression of TSPO mRNA and protein, reflecting findings in human carotid atherosclerosis.
Original languageEnglish
Pages (from-to)603-613
Number of pages11
JournalClinical Science
Volume127
Issue number10
DOIs
Publication statusPublished - Nov 2014

Fingerprint

Macrophages
Cholesterol
Phenotype
Lipids
Proteins
Carotid Artery Diseases
Apolipoprotein A-I
HDL Lipoproteins
Immunoblotting
Lipoproteins
Ligands
Polymerase Chain Reaction
Messenger RNA
Serum
Therapeutics

Keywords

  • macrophage
  • proteins
  • cholesterol

Cite this

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Targeting mitochondrial 18 kDa translocator protein (TSPO) regulates macrophage cholesterol efflux and lipid phenotype. / Taylor, Janice; Allen, Anne Marie; Graham, Ann.

In: Clinical Science, Vol. 127, No. 10, 11.2014, p. 603-613.

Research output: Contribution to journalArticle

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