Sampling area selection for the assessment of goblet cell density from conjunctival impression cytology specimens

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The aim of this study was to assess the impact of using different sampling areas (fields of view) on the reliability of goblet cell density (GCD) estimates from conjunctival impression cytology (CIC) specimens from healthy individuals. The CIC specimens were collected from the exposed nasal bulbar conjunctiva of 5 adult subjects (average age, 23 years) onto Biopore (Millicell) membranes and stained with Giemsa. A region from each of the specimens that contained abundant goblet cells was examined by light microscopy using a ×40 magnification objective lens, ×20 and ×10 lenses, the images were enlarged, and the goblet cells were marked and counted. The GCD values per square millimeter were calculated and then the impact of counting between 10 to many and 10 to few goblet cells assessed.

Original languageEnglish
Pages (from-to)122-129
Number of pages8
JournalEye and Contact Lens: Science and Clinical Practice
Volume38
Issue number2
DOIs
Publication statusPublished - Mar 2012

Keywords

  • ophthalmology
  • vision science
  • goblet cell density

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