Reverse genetics of Drosophila brain structure and function

J. W. Sentry, S. F. Goodwin, C. D. Milligan, A. Duncanson, M. Yang, K. Kaiser*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A set of molecular genetic technologies are described, which will have far reaching consequences for the study of brain structure, function and development in Drosophila melanogaster. Site selected mutagenesis (a PCR-based screen for P-element insertion events) allows insertion mutants to be isolated for any cloned gene, and is being used in this laboratory to ask questions about the rolls of particular cellular components in learning and memory. Transposants have been isolated in genes encoding a regulatory (RI) and a catalytic (DCO) subunit of cAMP-dependent protein kinase, and in a gene encoding a Gi-like alpha subunit. The alternative use of I factors is described. The PKA RI homozygous mutants display a significant decrement in initial learning ability. Enhancer-trap strategies, for which the GAL-4 P-element system is particularly convenient, allow the identification of genes expressed in the developing fly brain. Strategies for the efficient detection of such events are described.

Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalProgress in Neurobiology
Volume42
Issue number2
DOIs
Publication statusPublished - Feb 1994
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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