Reliability of nucleus-to-cell and nucleus-to-cytoplasm calculations for conjunctival impression cytology specimens

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The study aim was to assess the outcome of two different nucleo-cytoplasmic ratio calculations in relation to the number of cells measured from conjunctival impression cytology (CIC) images. CIC from the exposed nasal bulbar conjunctiva surface was undertaken on 40 young adults using a Millcell(®)-CM filter; the filters were stained with Giemsa, colour images taken of the cells in a monolayer at 200 X magnification, graded by the Nelson scale and then a projection overlay method used to outline the cell and nucleus borders. Nucleo-cytoplasmic ratios were calculated based on literal interpretation of the term (i.e. N/CYT¿=¿nucleus area/[cell area - nucleus area]) as well as based on cell area (i.e. N/CELL¿=¿nucleus area/cell area).
Original languageEnglish
Pages (from-to)583-591
Number of pages9
JournalCurrent Eye Research
Volume37
Issue number7
Early online date10 May 2012
DOIs
Publication statusPublished - Jul 2012

Keywords

  • ophthalmology
  • vision science
  • cytology

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