Rapid method to determine the molecular weight of dextrins and dextrans

Kamran Alvani; Xin Qi; Richard F. Tester

doi:10.1016/j.carbpol.2009.06.021

Rapid method to determine the molecular weight of dextrins and dextrans

Kamran Alvani, Xin Qi, Richard F. Tester

Biological and Biomedical Sciences

Research output: Contribution to journal › Article

Abstract

A rapid method was developed to determine the molecular weight (M_n) of ß-limit dextrin and dextrans (Leuconostoc mesenteroides) using a reducing power approach. The M_n of the ß-limit dextrin was also estimated from high performance liquid chromatography (HPLC). Chromatograms were pre-calibrated with the dextrans. The three dextrins had a M_n of 2.09, 2.40 and 2.63 × 10⁵ using the reducing method and 4.80, 5.90 and 2.80 × 10⁵ by HPLC. The method could be employed to estimate M_n of dextrins where chromatographic systems were not available.

Original language	English
Pages (from-to)	997-998
Number of pages	2
Journal	Carbohydrate Polymers
Volume	78
Issue number	4
DOIs	https://doi.org/10.1016/j.carbpol.2009.06.021
Publication status	Published - 1 Nov 2009

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Keywords

dextrans
food science
dextrins

Cite this

Alvani, K., Qi, X., & Tester, R. F. (2009). Rapid method to determine the molecular weight of dextrins and dextrans. Carbohydrate Polymers, 78(4), 997-998. https://doi.org/10.1016/j.carbpol.2009.06.021

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title = "Rapid method to determine the molecular weight of dextrins and dextrans",

abstract = "A rapid method was developed to determine the molecular weight (Mn) of {\ss}-limit dextrin and dextrans (Leuconostoc mesenteroides) using a reducing power approach. The Mn of the {\ss}-limit dextrin was also estimated from high performance liquid chromatography (HPLC). Chromatograms were pre-calibrated with the dextrans. The three dextrins had a Mn of 2.09, 2.40 and 2.63 × 105 using the reducing method and 4.80, 5.90 and 2.80 × 105 by HPLC. The method could be employed to estimate Mn of dextrins where chromatographic systems were not available.",

keywords = "dextrans, food science, dextrins",

author = "Kamran Alvani and Xin Qi and Tester, {Richard F.}",

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T1 - Rapid method to determine the molecular weight of dextrins and dextrans

AU - Alvani, Kamran

AU - Qi, Xin

AU - Tester, Richard F.

N1 - Originally published in: Carbohydrate Polymers (2009), 78 (4), pp.997-998.<a href="http://www.sciencedirect.com/science/journal/01448617" id="x-ddJrnl"> </a>

PY - 2009/11/1

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N2 - A rapid method was developed to determine the molecular weight (Mn) of ß-limit dextrin and dextrans (Leuconostoc mesenteroides) using a reducing power approach. The Mn of the ß-limit dextrin was also estimated from high performance liquid chromatography (HPLC). Chromatograms were pre-calibrated with the dextrans. The three dextrins had a Mn of 2.09, 2.40 and 2.63 × 105 using the reducing method and 4.80, 5.90 and 2.80 × 105 by HPLC. The method could be employed to estimate Mn of dextrins where chromatographic systems were not available.

AB - A rapid method was developed to determine the molecular weight (Mn) of ß-limit dextrin and dextrans (Leuconostoc mesenteroides) using a reducing power approach. The Mn of the ß-limit dextrin was also estimated from high performance liquid chromatography (HPLC). Chromatograms were pre-calibrated with the dextrans. The three dextrins had a Mn of 2.09, 2.40 and 2.63 × 105 using the reducing method and 4.80, 5.90 and 2.80 × 105 by HPLC. The method could be employed to estimate Mn of dextrins where chromatographic systems were not available.

KW - dextrans

KW - food science

KW - dextrins

U2 - 10.1016/j.carbpol.2009.06.021

DO - 10.1016/j.carbpol.2009.06.021

M3 - Article

VL - 78

SP - 997

EP - 998

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 4

ER -

Documents and Links

10.1016/j.carbpol.2009.06.021

http://researchonline.gcu.ac.uk/sls/321