Raf kinase inhibitor protein regulation of raf and MAPK signaling

Suzanne Hagan, Rey Garcia, Amardeep Dhillon, Walter Kolch

Research output: Contribution to journalReview articlepeer-review

45 Citations (Scopus)


The Raf kinase inhibitor protein (RKIP) belongs to an evolutionarily conserved family of phosphatidylethanolamine-binding proteins (PEBPs), which have important functions as inhibitors of kinase signaling pathways and metastasis. Most notably, RKIP can interrupt signaling through the Ras-Raf-MEK-ERK pathway by dissociating the interaction between Raf-1 and its substrate MEK, highlighting the importance of protein interactions as regulatory interfaces. Furthermore, RKIP was shown to inhibit IκB kinases (IKKs) interfering with the activation of nuclear factor kappa B (NFκB), and G-protein coupled receptor-kinase 2 (GRK2), impeding receptor downregulation and prolonging signaling. More recently, RKIP has emerged as an important suppressor of metastasis. Here, we review the functions of RKIP and present methods to detect and measure RKIP expression and activity in cells and tissues.

Original languageEnglish
Pages (from-to)248-259
Number of pages12
JournalMethods in Enzymology
Publication statusPublished - 6 Jun 2006

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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