Quantification of the influence of drugs on zebrafish larvae swimming kinematics and energetics

Zhenkai Zhao, Gen Li, Qing Xiao, Hui-Rong Jiang, Gabriel Mbuta Tchivelekete, Xinhua Shu, Hao Liu

Research output: Contribution to journalArticle

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Abstract

The use of zebrafish larvae has aroused wide interest in the medical field for its potential role in the development of new therapies. The larvae grow extremely quickly and the embryos are nearly transparent which allows easy examination of its internal structures using fluorescent imaging techniques. Medical treatment of zebrafish larvae can directly influence its swimming behaviours. These behaviour changes are related to functional changes of central nervous system and transformations of the zebrafish body such as muscle mechanical power and force variation, which cannot be measured directly by pure experiment observation. To quantify the influence of drugs on zebrafish larvae swimming behaviours and energetics, we have developed a novel methodology to exploit intravital changes based on observed zebrafish locomotion. Specifically, by using an in-house MATLAB code to process the recorded live zebrafish swimming video, the kinematic locomotion equation of a 3D zebrafish larvae was obtained, and a customised Computational Fluid Dynamics tool was used to solve the fluid flow around the fish model which was geometrically the same as experimentally tested zebrafish. The developed methodology was firstly verified against experiment, and further applied to quantify the fish internal body force, torque and power consumption associated with a group of normal zebrafish larvae vs. those immersed in acetic acid and two neuroactive drugs. As indicated by our results, zebrafish larvae immersed in 0.01% acetic acid display approximately 30% higher hydrodynamic power and 10% higher cost of transport than control group. In addition, 500 μM diphenylhydantoin significantly decreases the locomotion activity for approximately 50% lower hydrodynamic power, whereas 100 mg/L yohimbine has not caused any significant influences on 5 dpf zebrafish larvae locomotion. The approach has potential to evaluate the influence of drugs on the aquatic animal’s behaviour changes and thus support the development of new analgesic and neuroactive drugs.
Original languageEnglish
Number of pages26
JournalPeerJ
Volume8
DOIs
Publication statusPublished - 8 Jan 2020

Fingerprint

Kinematics
Acetic acid
Fish
Hydrodynamics
Neurology
MATLAB
Muscle
Flow of fluids
Computational fluid dynamics
Animals
Electric power utilization
Torque
Experiments
Imaging techniques
Swimming
Costs

Keywords

  • zebrafish
  • swimming behaviour
  • acetic acid
  • MBDyn
  • fluid-structure interaction
  • power
  • neuroactive
  • drug influence

Cite this

Zhao, Z., Li, G., Xiao, Q., Jiang, H-R., Tchivelekete, G. M., Shu, X., & Liu, H. (2020). Quantification of the influence of drugs on zebrafish larvae swimming kinematics and energetics. PeerJ, 8. https://doi.org/10.7717/peerj.8374
Zhao, Zhenkai ; Li, Gen ; Xiao, Qing ; Jiang, Hui-Rong ; Tchivelekete, Gabriel Mbuta ; Shu, Xinhua ; Liu, Hao. / Quantification of the influence of drugs on zebrafish larvae swimming kinematics and energetics. In: PeerJ. 2020 ; Vol. 8.
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Quantification of the influence of drugs on zebrafish larvae swimming kinematics and energetics. / Zhao, Zhenkai ; Li, Gen; Xiao, Qing ; Jiang, Hui-Rong; Tchivelekete, Gabriel Mbuta; Shu, Xinhua; Liu, Hao.

In: PeerJ, Vol. 8, 08.01.2020.

Research output: Contribution to journalArticle

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AU - Xiao, Qing

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