Pyrosequencing of Mytilus galloprovincialis cDNAs: tissue-specific expression patterns

John A. Craft, Jack A. Gilbert, Ben Temperton, Kate E. Dempsey, Kevin Ashelford, Bela Tiwari, Tom H. Hutchinson, Kevin Chipman

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Abstract

Mytilus species are important in marine ecology and in environmental quality assessment, yet their molecular biology is poorly understood. Molecular aspects of their reproduction, hybridisation between species, mitochondrial inheritance, skewed sex ratios of offspring and adaptation to climatic and pollution factors are priority areas. To start to address this situation, expressed genetic transcripts from M. galloprovincialis were pyrosequenced. Transcripts were isolated from the digestive gland, foot, gill and mantle of both male and female mussels. In total, 175,547 sequences were obtained and for foot and mantle, 90% of the sequences could be assembled into contiguous fragments but this reduced to 75% for the digestive gland and gill.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalPLoS ONE
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 2010

Keywords

  • tissue-specific expression
  • pyrosequencing
  • Mytilus galloprovincialis
  • transcriptome

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