Protection against oxidative stress by vitamin D in cone cells

Ali Mohammad Tohari, Xinzhi Zhou, Xinhua Shu

Research output: Contribution to journalArticle

Abstract

Photoreceptor degeneration (PD) refers to a group of heterogeneous outer retinal dystrophies characterized by the death of photoreceptors. Both oxidative stress and inflammation are involved in the pathogenesis of PD. We investigate whether vitamin D has a potential for the treatment of PD by evaluating the anti-oxidative stress and anti-inflammatory properties of the active form of vitamin D3, 1,a, 25-dihydroxyvitamin D3, in a mouse cone cell line, 661W. Mouse cone cells were treated with H2O2 or a mixture of H2O2 and vitamin D; cell viability was determined. The production of reactive oxygen species (ROS) in treated and untreated cells was measured. The expression of key anti-oxidative stress and inflammatory genes in treated and untreated cells was determined. Treatment with vitamin D significantly increased cell viability and decreased ROS production in 661W cells under oxidative stress induced by H2O2. H2O2 treatment in 661W cells can significantly down-regulate the expression of antioxidant genes and up-regulate the expression of neurotoxic cytokines. Vitamin D treatment significantly reversed these effects and restored the expression of antioxidant genes. Vitamin D treatment also can block H2O2 induced oxidative damages. The data suggested that vitamin D may offer a therapeutic potential for patients with PD.
Original languageEnglish
Pages (from-to)82-94
Number of pages13
JournalCell Biochemistry and Function
Volume34
Issue number2
Early online date17 Feb 2016
DOIs
Publication statusPublished - Mar 2016

Keywords

  • photoreceptor degeneration
  • retinal dystrophies
  • vitamin D

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