Potential therapeutic efficacy of photodynamic therapy on triple negative breast cancer in hormonal microenvironment

ESM Chu; GMN Leung; KCN Cheung; RWK Wu; John W. Yuen; Zheng  Huang

doi:10.1016/j.pdpdt.2024.104186

Abstract

Significance
There are seldom studies on the determination of cancer treatment efficacy related to normal hormonal tumor microenvironment on triple negative breast cancer (TNBC). This study aimed to determine Hexyl-ALA-PDT efficacy on TNBC in a simulated hormonal microenvironment.
Approach
The 3D spheroids of TNBC cells (MDA-MB-231) were generated in the hormonal supplemented microenvironment. The accumulation of PpIX, phototoxicity, the ROS level and p-ULK1 expression level mediated by Hexyl-ALA-PDT were determined by confocal microscopy, MTT assay and flow cytometry.
Results
A lower Hexyl-ALA concentration was required to achieve the same lethal dose of LD50 with hormonal supplement in 3D spheroids. It was found that the ROS level was increased at lower Hexyl-ALA-PDT dose in hormonal microenvironment, which also correlated the decrease of p-ULK1 expression by PDT.
Conclusions
Hexyl-ALA-PDT increased ROS level in TNBC in hormonal microenvironment; and with the reduced p-ULK1 expression thus indicating autophagy of cell death might be triggered.

Original language	English
Article number	104186
Number of pages	2
Journal	Photodiagnosis and Photodynamic Therapy
Volume	46
DOIs	https://doi.org/10.1016/j.pdpdt.2024.104186
Publication status	Published - 28 May 2024

Keywords

photodynamic therapy
triple negative breast cancer
hormonal microenvironment
3D spheroids
Hexyl-ALA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Documents and Links

10.1016/j.pdpdt.2024.104186

Final published versionFinal published version, 176 KBLicence: CC BY-NC-ND

Cite this

@article{ff80dbcbbe0a49be9ad5575363ac4ea6,

title = "Potential therapeutic efficacy of photodynamic therapy on triple negative breast cancer in hormonal microenvironment",

abstract = "SignificanceThere are seldom studies on the determination of cancer treatment efficacy related to normal hormonal tumor microenvironment on triple negative breast cancer (TNBC). This study aimed to determine Hexyl-ALA-PDT efficacy on TNBC in a simulated hormonal microenvironment.ApproachThe 3D spheroids of TNBC cells (MDA-MB-231) were generated in the hormonal supplemented microenvironment. The accumulation of PpIX, phototoxicity, the ROS level and p-ULK1 expression level mediated by Hexyl-ALA-PDT were determined by confocal microscopy, MTT assay and flow cytometry.ResultsA lower Hexyl-ALA concentration was required to achieve the same lethal dose of LD50 with hormonal supplement in 3D spheroids. It was found that the ROS level was increased at lower Hexyl-ALA-PDT dose in hormonal microenvironment, which also correlated the decrease of p-ULK1 expression by PDT.ConclusionsHexyl-ALA-PDT increased ROS level in TNBC in hormonal microenvironment; and with the reduced p-ULK1 expression thus indicating autophagy of cell death might be triggered.",

keywords = "photodynamic therapy, triple negative breast cancer, hormonal microenvironment, 3D spheroids, Hexyl-ALA",

author = "ESM Chu and GMN Leung and KCN Cheung and RWK Wu and Yuen, {John W.} and Zheng Huang",

year = "2024",

month = may,

day = "28",

doi = "10.1016/j.pdpdt.2024.104186",

language = "English",

volume = "46",

journal = "Photodiagnosis and Photodynamic Therapy",

issn = "1572-1000",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Potential therapeutic efficacy of photodynamic therapy on triple negative breast cancer in hormonal microenvironment

AU - Chu, ESM

AU - Leung, GMN

AU - Cheung, KCN

AU - Wu, RWK

AU - Yuen, John W.

AU - Huang, Zheng

PY - 2024/5/28

Y1 - 2024/5/28

N2 - SignificanceThere are seldom studies on the determination of cancer treatment efficacy related to normal hormonal tumor microenvironment on triple negative breast cancer (TNBC). This study aimed to determine Hexyl-ALA-PDT efficacy on TNBC in a simulated hormonal microenvironment.ApproachThe 3D spheroids of TNBC cells (MDA-MB-231) were generated in the hormonal supplemented microenvironment. The accumulation of PpIX, phototoxicity, the ROS level and p-ULK1 expression level mediated by Hexyl-ALA-PDT were determined by confocal microscopy, MTT assay and flow cytometry.ResultsA lower Hexyl-ALA concentration was required to achieve the same lethal dose of LD50 with hormonal supplement in 3D spheroids. It was found that the ROS level was increased at lower Hexyl-ALA-PDT dose in hormonal microenvironment, which also correlated the decrease of p-ULK1 expression by PDT.ConclusionsHexyl-ALA-PDT increased ROS level in TNBC in hormonal microenvironment; and with the reduced p-ULK1 expression thus indicating autophagy of cell death might be triggered.

AB - SignificanceThere are seldom studies on the determination of cancer treatment efficacy related to normal hormonal tumor microenvironment on triple negative breast cancer (TNBC). This study aimed to determine Hexyl-ALA-PDT efficacy on TNBC in a simulated hormonal microenvironment.ApproachThe 3D spheroids of TNBC cells (MDA-MB-231) were generated in the hormonal supplemented microenvironment. The accumulation of PpIX, phototoxicity, the ROS level and p-ULK1 expression level mediated by Hexyl-ALA-PDT were determined by confocal microscopy, MTT assay and flow cytometry.ResultsA lower Hexyl-ALA concentration was required to achieve the same lethal dose of LD50 with hormonal supplement in 3D spheroids. It was found that the ROS level was increased at lower Hexyl-ALA-PDT dose in hormonal microenvironment, which also correlated the decrease of p-ULK1 expression by PDT.ConclusionsHexyl-ALA-PDT increased ROS level in TNBC in hormonal microenvironment; and with the reduced p-ULK1 expression thus indicating autophagy of cell death might be triggered.

KW - photodynamic therapy

KW - triple negative breast cancer

KW - hormonal microenvironment

KW - 3D spheroids

KW - Hexyl-ALA

U2 - 10.1016/j.pdpdt.2024.104186

DO - 10.1016/j.pdpdt.2024.104186

M3 - Meeting abstract

SN - 1572-1000

VL - 46

JO - Photodiagnosis and Photodynamic Therapy

JF - Photodiagnosis and Photodynamic Therapy

M1 - 104186

ER -