Abstract
Previously a strategy for monitoring of pigs intended for cell transplantation was developed and successfully applied to several representative herds in New Zealand. A designated pathogen-free (DPF) herd has been chosen as a good candidate for xenotransplantation. This herd has previously tested free of infectious agents relevant to xenotransplantation and we present here an in depth study of porcine endogenous retrovirus (PERV) transmission. A panel of assays that describes the constraints for the transmission of PERV has been suggested. It includes a) infectivity test in coculture of DPF pig primary cells with both human and pig target cell lines; b) RT activity in supernatant of stimulated primary cells from DPF pigs; c) viral load in donor's blood plasma; d) PERV proviral copy number in DPF pig genome; e) PERV class C prevalence in the herd and its recombination potential.
| Original language | English |
|---|---|
| Pages (from-to) | 1381-1388 |
| Number of pages | 8 |
| Journal | Cell Transplantation |
| Volume | 17 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 1 Jan 2008 |
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Keywords
- retrovirus
- cell transplantation
- pigs
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Porcine endogenous retrovirus (PERV) and its transmission characteristics: a study of the New Zealand designated pathogen-free herd. / Garkavenko, O.; Wynyard, S.; Nathu, D.; Simond, D.; Muzina, M.; Muzina, Z.; Scobie, Linda; Hector, R.
In: Cell Transplantation, Vol. 17, No. 12, 01.01.2008, p. 1381-1388.Research output: Contribution to journal › Article
TY - JOUR
T1 - Porcine endogenous retrovirus (PERV) and its transmission characteristics: a study of the New Zealand designated pathogen-free herd
AU - Garkavenko, O.
AU - Wynyard, S.
AU - Nathu, D.
AU - Simond, D.
AU - Muzina, M.
AU - Muzina, Z.
AU - Scobie, Linda
AU - Hector, R.
N1 - Originally published in: Cell Transplantation (2008), 17 (12), pp.1381-1388.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Previously a strategy for monitoring of pigs intended for cell transplantation was developed and successfully applied to several representative herds in New Zealand. A designated pathogen-free (DPF) herd has been chosen as a good candidate for xenotransplantation. This herd has previously tested free of infectious agents relevant to xenotransplantation and we present here an in depth study of porcine endogenous retrovirus (PERV) transmission. A panel of assays that describes the constraints for the transmission of PERV has been suggested. It includes a) infectivity test in coculture of DPF pig primary cells with both human and pig target cell lines; b) RT activity in supernatant of stimulated primary cells from DPF pigs; c) viral load in donor's blood plasma; d) PERV proviral copy number in DPF pig genome; e) PERV class C prevalence in the herd and its recombination potential.
AB - Previously a strategy for monitoring of pigs intended for cell transplantation was developed and successfully applied to several representative herds in New Zealand. A designated pathogen-free (DPF) herd has been chosen as a good candidate for xenotransplantation. This herd has previously tested free of infectious agents relevant to xenotransplantation and we present here an in depth study of porcine endogenous retrovirus (PERV) transmission. A panel of assays that describes the constraints for the transmission of PERV has been suggested. It includes a) infectivity test in coculture of DPF pig primary cells with both human and pig target cell lines; b) RT activity in supernatant of stimulated primary cells from DPF pigs; c) viral load in donor's blood plasma; d) PERV proviral copy number in DPF pig genome; e) PERV class C prevalence in the herd and its recombination potential.
KW - retrovirus
KW - cell transplantation
KW - pigs
U2 - 10.3727/096368908787648056
DO - 10.3727/096368908787648056
M3 - Article
VL - 17
SP - 1381
EP - 1388
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 12
ER -