Porcine endogenous retrovirus (PERV) and its transmission characteristics: a study of the New Zealand designated pathogen-free herd

O. Garkavenko, S. Wynyard, D. Nathu, D. Simond, M. Muzina, Z. Muzina, Linda Scobie, R. Hector

Research output: Contribution to journalArticle

Abstract

Previously a strategy for monitoring of pigs intended for cell transplantation was developed and successfully applied to several representative herds in New Zealand. A designated pathogen-free (DPF) herd has been chosen as a good candidate for xenotransplantation. This herd has previously tested free of infectious agents relevant to xenotransplantation and we present here an in depth study of porcine endogenous retrovirus (PERV) transmission. A panel of assays that describes the constraints for the transmission of PERV has been suggested. It includes a) infectivity test in coculture of DPF pig primary cells with both human and pig target cell lines; b) RT activity in supernatant of stimulated primary cells from DPF pigs; c) viral load in donor's blood plasma; d) PERV proviral copy number in DPF pig genome; e) PERV class C prevalence in the herd and its recombination potential.

Original languageEnglish
Pages (from-to)1381-1388
Number of pages8
JournalCell Transplantation
Volume17
Issue number12
DOIs
Publication statusPublished - 1 Jan 2008

Keywords

  • retrovirus
  • cell transplantation
  • pigs

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