Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov

Niall A. Logan; G. Forsyth; L. Lebbe; J. Goris; M. Heyndrickx; A. Balcaen; A. Verhelst; E. Falsen

doi:10.1099/ijs.0.02081-0

Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov

Niall A. Logan, G. Forsyth, L. Lebbe, J. Goris, M. Heyndrickx, A. Balcaen, A. Verhelst, E. Falsen

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA--DNA reassociation, and phenotypically by using fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of Bacillus badius, 12 as Brevibacillus agri, including 3 strains associated with an outbreak of water-borne illness, 4 as Brevibacillus centrosporus and 2 as Brevibacillus parabrevis; 12 strains contaminating an antibiotic production plant were recognized as members of a new species, for which the name Brevibacillus invocatus is proposed, with the type strain LMG 18962(T) (=B2156(T)=CIP 106911(T)=NCIMB 13772(T)).

Original language	English
Journal	International Journal of Systematic and Evolutionary Microbiology
DOIs	https://doi.org/10.1099/ijs.0.02081-0
Publication status	Published - May 2002

Keywords

endospores
Brevibacillus
aerobic bateria
Aneurinibacillus
16S rDNA sequencing
Bacillus

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10.1099/ijs.0.02081-0

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Logan, N. A., Forsyth, G., Lebbe, L., Goris, J., Heyndrickx, M., Balcaen, A., Verhelst, A., & Falsen, E. (2002). Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov. International Journal of Systematic and Evolutionary Microbiology. https://doi.org/10.1099/ijs.0.02081-0

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title = "Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov",

abstract = "Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA--DNA reassociation, and phenotypically by using fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of Bacillus badius, 12 as Brevibacillus agri, including 3 strains associated with an outbreak of water-borne illness, 4 as Brevibacillus centrosporus and 2 as Brevibacillus parabrevis; 12 strains contaminating an antibiotic production plant were recognized as members of a new species, for which the name Brevibacillus invocatus is proposed, with the type strain LMG 18962(T) (=B2156(T)=CIP 106911(T)=NCIMB 13772(T)).",

keywords = "endospores, Brevibacillus, aerobic bateria, Aneurinibacillus, 16S rDNA sequencing, Bacillus",

author = "Logan, {Niall A.} and G. Forsyth and L. Lebbe and J. Goris and M. Heyndrickx and A. Balcaen and A. Verhelst and E. Falsen",

note = "Originally published in: International Journal of Systematic and Evolutionary Microbiology (2002), 52 (3), pp.953-966. Publisher version not permitted in repositories. ",

year = "2002",

month = may,

doi = "10.1099/ijs.0.02081-0",

language = "English",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

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TY - JOUR

T1 - Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov

AU - Logan, Niall A.

AU - Forsyth, G.

AU - Lebbe, L.

AU - Goris, J.

AU - Heyndrickx, M.

AU - Balcaen, A.

AU - Verhelst, A.

AU - Falsen, E.

N1 - Originally published in: International Journal of Systematic and Evolutionary Microbiology (2002), 52 (3), pp.953-966. Publisher version not permitted in repositories.

PY - 2002/5

Y1 - 2002/5

N2 - Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA--DNA reassociation, and phenotypically by using fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of Bacillus badius, 12 as Brevibacillus agri, including 3 strains associated with an outbreak of water-borne illness, 4 as Brevibacillus centrosporus and 2 as Brevibacillus parabrevis; 12 strains contaminating an antibiotic production plant were recognized as members of a new species, for which the name Brevibacillus invocatus is proposed, with the type strain LMG 18962(T) (=B2156(T)=CIP 106911(T)=NCIMB 13772(T)).

AB - Thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rDNA restriction analysis (ARDRA), 16S rDNA sequencing and DNA--DNA reassociation, and phenotypically by using fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins, API Biotype 100 assimilation tests and 16 other routine phenotypic tests. Three isolates were identified as strains of Bacillus badius, 12 as Brevibacillus agri, including 3 strains associated with an outbreak of water-borne illness, 4 as Brevibacillus centrosporus and 2 as Brevibacillus parabrevis; 12 strains contaminating an antibiotic production plant were recognized as members of a new species, for which the name Brevibacillus invocatus is proposed, with the type strain LMG 18962(T) (=B2156(T)=CIP 106911(T)=NCIMB 13772(T)).

KW - endospores

KW - Brevibacillus

KW - aerobic bateria

KW - Aneurinibacillus

KW - 16S rDNA sequencing

KW - Bacillus

U2 - 10.1099/ijs.0.02081-0

DO - 10.1099/ijs.0.02081-0

M3 - Article

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

ER -

Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov

Abstract

Keywords

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