Polymicrobial oral biofilm models: simplifying the complex

Jason L Brown, William Johnston, Christopher Delaney, Bryn Short, Mark C Butcher, Tracy Young, John Butcher, Marcello Riggio, Shauna Culshaw, Gordon Ramage

Research output: Contribution to journalLiterature review

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Abstract

Over the past century, numerous studies have used oral biofilm models to investigate growth kinetics, biofilm formation, structure and composition, antimicrobial susceptibility and host-pathogen interactions. In vivo animal models provide useful models of some oral diseases; however, these are expensive and carry vast ethical implications. Oral biofilms grown or maintained in vitro offer a useful platform for certain studies and have the advantages of being inexpensive to establish and easy to reproduce and manipulate. In addition, a wide range of variables can be monitored and adjusted to mimic the dynamic environmental changes at different sites in the oral cavity, such as pH, temperature, salivary and gingival crevicular fluid flow rates, or microbial composition. This review provides a detailed insight for early-career oral science researchers into how the biofilm models used in oral research have progressed and improved over the years, their advantages and disadvantages, and how such systems have contributed to our current understanding of oral disease pathogenesis and aetiology.
Original languageEnglish
Pages (from-to)1573-1584
Number of pages12
JournalJournal of Medical Microbiology
Volume68
Issue number11
Early online date1 Nov 2019
DOIs
Publication statusE-pub ahead of print - 1 Nov 2019

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Biofilms
Mouth Diseases
Gingival Crevicular Fluid
Host-Pathogen Interactions
Mouth
Animal Models
Research Personnel
Temperature
Growth
Research

Keywords

  • biofilms
  • dental plaque
  • host–pathogen interactions
  • oral biofilm models
  • oral microbiology

Cite this

Brown, J. L., Johnston, W., Delaney, C., Short, B., Butcher, M. C., Young, T., ... Ramage, G. (2019). Polymicrobial oral biofilm models: simplifying the complex. Journal of Medical Microbiology, 68(11), 1573-1584. https://doi.org/10.1099/jmm.0.001063
Brown, Jason L ; Johnston, William ; Delaney, Christopher ; Short, Bryn ; Butcher, Mark C ; Young, Tracy ; Butcher, John ; Riggio, Marcello ; Culshaw, Shauna ; Ramage, Gordon. / Polymicrobial oral biofilm models: simplifying the complex. In: Journal of Medical Microbiology. 2019 ; Vol. 68, No. 11. pp. 1573-1584.
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abstract = "Over the past century, numerous studies have used oral biofilm models to investigate growth kinetics, biofilm formation, structure and composition, antimicrobial susceptibility and host-pathogen interactions. In vivo animal models provide useful models of some oral diseases; however, these are expensive and carry vast ethical implications. Oral biofilms grown or maintained in vitro offer a useful platform for certain studies and have the advantages of being inexpensive to establish and easy to reproduce and manipulate. In addition, a wide range of variables can be monitored and adjusted to mimic the dynamic environmental changes at different sites in the oral cavity, such as pH, temperature, salivary and gingival crevicular fluid flow rates, or microbial composition. This review provides a detailed insight for early-career oral science researchers into how the biofilm models used in oral research have progressed and improved over the years, their advantages and disadvantages, and how such systems have contributed to our current understanding of oral disease pathogenesis and aetiology.",
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Brown, JL, Johnston, W, Delaney, C, Short, B, Butcher, MC, Young, T, Butcher, J, Riggio, M, Culshaw, S & Ramage, G 2019, 'Polymicrobial oral biofilm models: simplifying the complex', Journal of Medical Microbiology, vol. 68, no. 11, pp. 1573-1584. https://doi.org/10.1099/jmm.0.001063

Polymicrobial oral biofilm models: simplifying the complex. / Brown, Jason L; Johnston, William; Delaney, Christopher; Short, Bryn; Butcher, Mark C; Young, Tracy; Butcher, John; Riggio, Marcello ; Culshaw, Shauna; Ramage, Gordon.

In: Journal of Medical Microbiology, Vol. 68, No. 11, 01.11.2019, p. 1573-1584.

Research output: Contribution to journalLiterature review

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AU - Short, Bryn

AU - Butcher, Mark C

AU - Young, Tracy

AU - Butcher, John

AU - Riggio, Marcello

AU - Culshaw, Shauna

AU - Ramage, Gordon

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Brown JL, Johnston W, Delaney C, Short B, Butcher MC, Young T et al. Polymicrobial oral biofilm models: simplifying the complex. Journal of Medical Microbiology. 2019 Nov 1;68(11):1573-1584. https://doi.org/10.1099/jmm.0.001063