Physiological and immunocytochemical evidence that glutamatergic neurotransmission is involved in the activation of arm autotomy in the featherstar Antedon mediterranea (Echinodermata: Crinoidea)

Iain Wilkie, A. Barbaglio, W. M. Maclaren, M. Daniela Candia Carnevali

Research output: Contribution to journalArticle

Abstract

The crinoid echinoderm Antedon mediterranea autotomises its arms at specialised skeletal joints known as syzygies that occur at regular intervals along the length of each arm. Detachment is achieved through the nervously mediated destabilisation of ligament fibres at a particular syzygy. The aim of this investigation was to identify neurotransmitters that are involved in the autotomy response. Physiological experiments were conducted on isolated preparations of syzygial joints, which can be induced to undergo autotomy-like fracture by applying stimulatory agents such as elevated [K+]o. Initial experiments with elevated [K+]o showed that the autotomy threshold (the minimum amount of stimulation required to provoke autotomy) is lowest in syzygies at the arm base and rises distally. Of a range of neurotransmitter agonists tested, only L-glutamate invoked syzygial destabilisation, as did its analogues L-aspartate, -amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) and kainate, but not L-(+)-2-amino-4-phosphonobutyrate (L-AP4) or N-methyl-D-aspartate (NMDA).

Original languageEnglish
Pages (from-to)2104-2115
Number of pages12
JournalJournal of Experimental Biology
Volume213
DOIs
Publication statusPublished - 1 Jan 2010

Fingerprint

Echinodermata
Crinoidea
autotomy
aspartic acid
neurotransmitters
joints (animal)
Synaptic Transmission
Neurotransmitter Agents
Joints
Isoxazoles
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Kainic Acid
Propionates
N-Methylaspartate
ligaments
propionates
Ligaments
Aspartic Acid
glutamates
agonists

Keywords

  • crinoid
  • autotomy
  • Antedon
  • juxtaligamental cells
  • glutamate

Cite this

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title = "Physiological and immunocytochemical evidence that glutamatergic neurotransmission is involved in the activation of arm autotomy in the featherstar Antedon mediterranea (Echinodermata: Crinoidea)",
abstract = "The crinoid echinoderm Antedon mediterranea autotomises its arms at specialised skeletal joints known as syzygies that occur at regular intervals along the length of each arm. Detachment is achieved through the nervously mediated destabilisation of ligament fibres at a particular syzygy. The aim of this investigation was to identify neurotransmitters that are involved in the autotomy response. Physiological experiments were conducted on isolated preparations of syzygial joints, which can be induced to undergo autotomy-like fracture by applying stimulatory agents such as elevated [K+]o. Initial experiments with elevated [K+]o showed that the autotomy threshold (the minimum amount of stimulation required to provoke autotomy) is lowest in syzygies at the arm base and rises distally. Of a range of neurotransmitter agonists tested, only L-glutamate invoked syzygial destabilisation, as did its analogues L-aspartate, -amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) and kainate, but not L-(+)-2-amino-4-phosphonobutyrate (L-AP4) or N-methyl-D-aspartate (NMDA).",
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author = "Iain Wilkie and A. Barbaglio and Maclaren, {W. M.} and {Candia Carnevali}, {M. Daniela}",
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Physiological and immunocytochemical evidence that glutamatergic neurotransmission is involved in the activation of arm autotomy in the featherstar Antedon mediterranea (Echinodermata: Crinoidea). / Wilkie, Iain; Barbaglio, A.; Maclaren, W. M.; Candia Carnevali, M. Daniela.

In: Journal of Experimental Biology, Vol. 213, 01.01.2010, p. 2104-2115.

Research output: Contribution to journalArticle

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AU - Wilkie, Iain

AU - Barbaglio, A.

AU - Maclaren, W. M.

AU - Candia Carnevali, M. Daniela

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N2 - The crinoid echinoderm Antedon mediterranea autotomises its arms at specialised skeletal joints known as syzygies that occur at regular intervals along the length of each arm. Detachment is achieved through the nervously mediated destabilisation of ligament fibres at a particular syzygy. The aim of this investigation was to identify neurotransmitters that are involved in the autotomy response. Physiological experiments were conducted on isolated preparations of syzygial joints, which can be induced to undergo autotomy-like fracture by applying stimulatory agents such as elevated [K+]o. Initial experiments with elevated [K+]o showed that the autotomy threshold (the minimum amount of stimulation required to provoke autotomy) is lowest in syzygies at the arm base and rises distally. Of a range of neurotransmitter agonists tested, only L-glutamate invoked syzygial destabilisation, as did its analogues L-aspartate, -amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) and kainate, but not L-(+)-2-amino-4-phosphonobutyrate (L-AP4) or N-methyl-D-aspartate (NMDA).

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