pH dependent spectral properties of sodium fluorescein ophthalmic solutions revisited

Michael Doughty

Research output: Contribution to journalArticle

Abstract

This study aimed to re-assess and further quantify the pH-related changes in the absorbance and fluorescence emission spectra of sodium fluorescein in buffered aqueous solutions. nalytical grade sodium fluorescein (NaF) or commercial NaF ophthalmic products (Minims® 2% sodium fluorescein or Fluorets® sodium fluorescein ophthalmic strips) were prepared over a range of dilutions in 1% NaCl with the pH set with phosphate, borate or acetate buffer mixtures between pH 4.0 and 10.0 at room temperature. Absorbance and fluorescence spectra were recorded in 10 mm pathlength cuvettes.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalOphthalmic and Physiological Optics
Volume30
Issue number2
DOIs
Publication statusPublished - 1 Mar 2010

Keywords

  • optics
  • opthalmology
  • vision sciences

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