Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica

Marina Rodriguez-Diaz; Liesbeth Lebbe; Belén Rodelas; Jeroen Heyrman; Paul De Vos; Niall A. Logan

doi:10.1099/ijs.0.63395-0

Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica

Marina Rodriguez-Diaz, Liesbeth Lebbe, Belén Rodelas, Jeroen Heyrman, Paul De Vos, Niall A. Logan

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 °C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176T=CIP 108306T).

Original language	English
Journal	International Journal of Systematic and Evolutionary Microbiology
DOIs	https://doi.org/10.1099/ijs.0.63395-0
Publication status	Published - Sept 2005

Keywords

Antarctica
endospores
anaerobic bacteria

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10.1099/ijs.0.63395-0

Cite this

@article{0358f137969441ac8224ee37eec1fccf,

title = "Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica",

abstract = "Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 °C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176T=CIP 108306T).",

keywords = "Antarctica, endospores, anaerobic bacteria",

author = "Marina Rodriguez-Diaz and Liesbeth Lebbe and Bel{\'e}n Rodelas and Jeroen Heyrman and {De Vos}, Paul and Logan, {Niall A.}",

note = "Originally published in: International Journal of Systematic and Evolutionary Microbiology (2005), 55 (5), pp.2093-2099. Publisher version not permitted in IRs ET 24-5-13",

year = "2005",

month = sep,

doi = "10.1099/ijs.0.63395-0",

language = "English",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5034",

publisher = "Microbiology Society",

}

TY - JOUR

T1 - Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica

AU - Rodriguez-Diaz, Marina

AU - Lebbe, Liesbeth

AU - Rodelas, Belén

AU - Heyrman, Jeroen

AU - De Vos, Paul

AU - Logan, Niall A.

N1 - Originally published in: International Journal of Systematic and Evolutionary Microbiology (2005), 55 (5), pp.2093-2099. Publisher version not permitted in IRs ET 24-5-13

PY - 2005/9

Y1 - 2005/9

N2 - Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 °C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176T=CIP 108306T).

AB - Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 °C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176T=CIP 108306T).

KW - Antarctica

KW - endospores

KW - anaerobic bacteria

U2 - 10.1099/ijs.0.63395-0

DO - 10.1099/ijs.0.63395-0

M3 - Article

SN - 1466-5034

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

ER -

Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica

Abstract

Keywords

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