Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica

Marina Rodriguez-Diaz, Liesbeth Lebbe, Belén Rodelas, Jeroen Heyrman, Paul De Vos, Niall A. Logan

Research output: Contribution to journalArticle

Abstract

Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 °C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176T=CIP 108306T).

Original languageEnglish
JournalInternational Journal of Systematic and Evolutionary Microbiology
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Paenibacillus
Islands
Gram-Negative Facultatively Anaerobic Rods
Antarctica
Endospore-Forming Bacteria
Soil
Sporangia
Mars
Nitrogenase
endospores
oases
gene
new species
sporangia
nitrogenase
Ribosomal DNA
Spores
rRNA Genes
Genes
Polyacrylamide Gel Electrophoresis

Keywords

  • Antarctica
  • endospores
  • anaerobic bacteria

Cite this

Rodriguez-Diaz, Marina ; Lebbe, Liesbeth ; Rodelas, Belén ; Heyrman, Jeroen ; De Vos, Paul ; Logan, Niall A. / Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica. In: International Journal of Systematic and Evolutionary Microbiology. 2005.
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abstract = "Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 °C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176T=CIP 108306T).",
keywords = "Antarctica, endospores, anaerobic bacteria",
author = "Marina Rodriguez-Diaz and Liesbeth Lebbe and Bel{\'e}n Rodelas and Jeroen Heyrman and {De Vos}, Paul and Logan, {Niall A.}",
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Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica. / Rodriguez-Diaz, Marina; Lebbe, Liesbeth; Rodelas, Belén; Heyrman, Jeroen; De Vos, Paul; Logan, Niall A.

In: International Journal of Systematic and Evolutionary Microbiology, 09.2005.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica

AU - Rodriguez-Diaz, Marina

AU - Lebbe, Liesbeth

AU - Rodelas, Belén

AU - Heyrman, Jeroen

AU - De Vos, Paul

AU - Logan, Niall A.

N1 - Originally published in: International Journal of Systematic and Evolutionary Microbiology (2005), 55 (5), pp.2093-2099. Publisher version not permitted in IRs ET 24-5-13

PY - 2005/9

Y1 - 2005/9

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AB - Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 °C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176T=CIP 108306T).

KW - Antarctica

KW - endospores

KW - anaerobic bacteria

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M3 - Article

JO - International Journal of Systematic and Evolutionary Microbiology

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SN - 1466-5026

ER -