On the use of NIH image J for objective assessment of conjunctival cell and nucleus dimensions of impression cytology samples

Michael Doughty

doi:10.1097/ICL.0b013e31820c6df0

On the use of NIH image J for objective assessment of conjunctival cell and nucleus dimensions of impression cytology samples

Michael Doughty

Vision Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The aim of this study was to assess the use of a public domain software Image J (NIH Image, Bethesda, MD) to make dimensional measures of human bulbar conjunctival cells. Impression cytology samples were obtained from the nasal bulbar conjunctiva, and color images were taken at 200× magnification and projected and an overlay prepared or the image uploaded into Image J. The final image magnification for the overlays was approximately 2× that on the computer screen. For either overlays or screen images, linear measures were made from 30 or 25 cells of the cell longest dimension (LONG) and of the longest dimension of the nucleus (NUCLONG). The predicted variability in measures, from the calculated average values for any particular image, was systematically assessed, and the overall average results were compared.

Original language	English
Pages (from-to)	50-56
Number of pages	7
Journal	Eye and Contact Lens: Science and Clinical Practice
Volume	37
Issue number	2
DOIs	https://doi.org/10.1097/ICL.0b013e31820c6df0
Publication status	Published - 1 Mar 2011

Keywords

optometry
cytology
vision sciences

Documents and Links

10.1097/ICL.0b013e31820c6df0

Cite this

@article{d7c403dfef8f43cca27733b223320c5a,

title = "On the use of NIH image J for objective assessment of conjunctival cell and nucleus dimensions of impression cytology samples",

abstract = "The aim of this study was to assess the use of a public domain software Image J (NIH Image, Bethesda, MD) to make dimensional measures of human bulbar conjunctival cells. Impression cytology samples were obtained from the nasal bulbar conjunctiva, and color images were taken at 200× magnification and projected and an overlay prepared or the image uploaded into Image J. The final image magnification for the overlays was approximately 2× that on the computer screen. For either overlays or screen images, linear measures were made from 30 or 25 cells of the cell longest dimension (LONG) and of the longest dimension of the nucleus (NUCLONG). The predicted variability in measures, from the calculated average values for any particular image, was systematically assessed, and the overall average results were compared.",

keywords = "optometry, cytology, vision sciences",

author = "Michael Doughty",

note = "<p>Originally published in: Eye and Contact Lens: Science and Clinical Practice (2011), 37 (2), pp.50-56.</p>",

year = "2011",

month = mar,

day = "1",

doi = "10.1097/ICL.0b013e31820c6df0",

language = "English",

volume = "37",

pages = "50--56",

journal = "Eye and Contact Lens: Science and Clinical Practice",

issn = "1542-233X)",

publisher = "Lippincott Williams and Wilkins Ltd.",

number = "2",

}

TY - JOUR

T1 - On the use of NIH image J for objective assessment of conjunctival cell and nucleus dimensions of impression cytology samples

AU - Doughty, Michael

N1 - <p>Originally published in: Eye and Contact Lens: Science and Clinical Practice (2011), 37 (2), pp.50-56.</p>

PY - 2011/3/1

Y1 - 2011/3/1

N2 - The aim of this study was to assess the use of a public domain software Image J (NIH Image, Bethesda, MD) to make dimensional measures of human bulbar conjunctival cells. Impression cytology samples were obtained from the nasal bulbar conjunctiva, and color images were taken at 200× magnification and projected and an overlay prepared or the image uploaded into Image J. The final image magnification for the overlays was approximately 2× that on the computer screen. For either overlays or screen images, linear measures were made from 30 or 25 cells of the cell longest dimension (LONG) and of the longest dimension of the nucleus (NUCLONG). The predicted variability in measures, from the calculated average values for any particular image, was systematically assessed, and the overall average results were compared.

AB - The aim of this study was to assess the use of a public domain software Image J (NIH Image, Bethesda, MD) to make dimensional measures of human bulbar conjunctival cells. Impression cytology samples were obtained from the nasal bulbar conjunctiva, and color images were taken at 200× magnification and projected and an overlay prepared or the image uploaded into Image J. The final image magnification for the overlays was approximately 2× that on the computer screen. For either overlays or screen images, linear measures were made from 30 or 25 cells of the cell longest dimension (LONG) and of the longest dimension of the nucleus (NUCLONG). The predicted variability in measures, from the calculated average values for any particular image, was systematically assessed, and the overall average results were compared.

KW - optometry

KW - cytology

KW - vision sciences

U2 - 10.1097/ICL.0b013e31820c6df0

DO - 10.1097/ICL.0b013e31820c6df0

M3 - Article

SN - 1542-233X)

VL - 37

SP - 50

EP - 56

JO - Eye and Contact Lens: Science and Clinical Practice

JF - Eye and Contact Lens: Science and Clinical Practice

IS - 2

ER -

On the use of NIH image J for objective assessment of conjunctival cell and nucleus dimensions of impression cytology samples

Abstract

Keywords

Documents and Links

Fingerprint

Cite this