On the use of NIH image J for objective assessment of conjunctival cell and nucleus dimensions of impression cytology samples

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The aim of this study was to assess the use of a public domain software Image J (NIH Image, Bethesda, MD) to make dimensional measures of human bulbar conjunctival cells. Impression cytology samples were obtained from the nasal bulbar conjunctiva, and color images were taken at 200× magnification and projected and an overlay prepared or the image uploaded into Image J. The final image magnification for the overlays was approximately 2× that on the computer screen. For either overlays or screen images, linear measures were made from 30 or 25 cells of the cell longest dimension (LONG) and of the longest dimension of the nucleus (NUCLONG). The predicted variability in measures, from the calculated average values for any particular image, was systematically assessed, and the overall average results were compared.

Original languageEnglish
Pages (from-to)50-56
Number of pages7
JournalEye and Contact Lens: Science and Clinical Practice
Volume37
Issue number2
DOIs
Publication statusPublished - 1 Mar 2011

Keywords

  • optometry
  • cytology
  • vision sciences

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