Objective assessment of conjunctival squamous metaplasia by measures of cell and nucleus dimensions

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The main aim of this work is to present a special method for conjunctival impression cytology (CIC) and objective measures of measure cell and nucleus dimensions. CIC using Millicell1 Biopore filters was obtained from 50 Caucasian adults from the nasal bulbar conjunctiva. Giemsa-stained images of cells in a monolayer were photographed with a scale
bar and projected at 1,5003 to generate an overlay. Measures were made of both the long (L) and short (S) dimensions of the cells and the longest dimension of the nucleus (LN). The selected images comprised sets of 10 from different individuals, which had been assigned grades 0, 1, 2, or 3 and an additional grade 0.5.

Original languageEnglish
Pages (from-to)409-423
Number of pages15
JournalDiagnostic Cytopathology
Volume39
Issue number6
DOIs
Publication statusPublished - 1 Jun 2011

Keywords

  • optometry
  • cytology
  • vision sciences

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