Objective assessment of conjunctival squamous metaplasia by measures of cell and nucleus dimensions

Michael Doughty

Research output: Contribution to journalArticle

Abstract

The main aim of this work is to present a special method for conjunctival impression cytology (CIC) and objective measures of measure cell and nucleus dimensions. CIC using Millicell® Biopore filters was obtained from 50 Caucasian adults from the nasal bulbar conjunctiva. Giemsa-stained images of cells in a monolayer were photographed with a scale bar and projected at 1,500× to generate an overlay. Measures were made of both the long (L) and short (S) dimensions of the cells and the longest dimension of the nucleus (LN). The selected images comprised sets of 10 from different individuals, which had been assigned grades 0, 1, 2, or 3 and an additional grade 0.5.

Original languageEnglish
JournalDiagnostic Cytopathology
DOIs
Publication statusPublished - 1 May 2010

Keywords

  • cytology
  • vision sciences

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