Multiparameter detection of apoptosis using red-excitable SYTO probes

Donald Wlodkowic; Joanna Skommer; Chris Hillier; Zbigniew Darzynkiewicz

doi:10.1002/cyto.a.20564

Multiparameter detection of apoptosis using red-excitable SYTO probes

Donald Wlodkowic, Joanna Skommer, Chris Hillier, Zbigniew Darzynkiewicz

Biological and Biomedical Sciences

Research output: Contribution to journal › Article

Abstract

Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.

Original language	English
Pages (from-to)	563-569
Number of pages	7
Journal	Cytometry: Part A
Volume	73A
Issue number	6
DOIs	https://doi.org/10.1002/cyto.a.20564
Publication status	Published - 1 Jun 2008

Keywords

apoptosis
flow cytometry
anticancer drugs

Documents and Links

10.1002/cyto.a.20564

http://researchonline.gcu.ac.uk/sls/260

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Wlodkowic, D., Skommer, J., Hillier, C., & Darzynkiewicz, Z. (2008). Multiparameter detection of apoptosis using red-excitable SYTO probes. Cytometry: Part A, 73A(6), 563-569. https://doi.org/10.1002/cyto.a.20564

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abstract = "Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.",

keywords = "apoptosis, flow cytometry, anticancer drugs",

author = "Donald Wlodkowic and Joanna Skommer and Chris Hillier and Zbigniew Darzynkiewicz",

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AU - Wlodkowic, Donald

AU - Skommer, Joanna

AU - Hillier, Chris

AU - Darzynkiewicz, Zbigniew

N1 - Originally published in: Cytometry: Part A (2008), 73A (6), pp.563-569.

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N2 - Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.

AB - Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.

KW - apoptosis

KW - flow cytometry

KW - anticancer drugs

U2 - 10.1002/cyto.a.20564

DO - 10.1002/cyto.a.20564

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EP - 569

JO - Cytometry: Part A

JF - Cytometry: Part A

SN - 1552-4922

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