Multiparameter detection of apoptosis using red-excitable SYTO probes

Donald Wlodkowic, Joanna Skommer, Chris Hillier, Zbigniew Darzynkiewicz

Research output: Contribution to journalArticle

Abstract

Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.

Original languageEnglish
Pages (from-to)563-569
Number of pages7
JournalCytometry: Part A
Volume73A
Issue number6
DOIs
Publication statusPublished - 1 Jun 2008

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Fluorescent Dyes
Apoptosis
Preclinical Drug Evaluations
Cell Death
Coloring Agents

Keywords

  • apoptosis
  • flow cytometry
  • anticancer drugs

Cite this

Wlodkowic, D., Skommer, J., Hillier, C., & Darzynkiewicz, Z. (2008). Multiparameter detection of apoptosis using red-excitable SYTO probes. Cytometry: Part A, 73A(6), 563-569. https://doi.org/10.1002/cyto.a.20564
Wlodkowic, Donald ; Skommer, Joanna ; Hillier, Chris ; Darzynkiewicz, Zbigniew. / Multiparameter detection of apoptosis using red-excitable SYTO probes. In: Cytometry: Part A. 2008 ; Vol. 73A, No. 6. pp. 563-569.
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Wlodkowic, D, Skommer, J, Hillier, C & Darzynkiewicz, Z 2008, 'Multiparameter detection of apoptosis using red-excitable SYTO probes', Cytometry: Part A, vol. 73A, no. 6, pp. 563-569. https://doi.org/10.1002/cyto.a.20564

Multiparameter detection of apoptosis using red-excitable SYTO probes. / Wlodkowic, Donald; Skommer, Joanna; Hillier, Chris; Darzynkiewicz, Zbigniew.

In: Cytometry: Part A, Vol. 73A, No. 6, 01.06.2008, p. 563-569.

Research output: Contribution to journalArticle

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T1 - Multiparameter detection of apoptosis using red-excitable SYTO probes

AU - Wlodkowic, Donald

AU - Skommer, Joanna

AU - Hillier, Chris

AU - Darzynkiewicz, Zbigniew

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AB - Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.

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KW - flow cytometry

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Wlodkowic D, Skommer J, Hillier C, Darzynkiewicz Z. Multiparameter detection of apoptosis using red-excitable SYTO probes. Cytometry: Part A. 2008 Jun 1;73A(6):563-569. https://doi.org/10.1002/cyto.a.20564