Multiparameter detection of apoptosis using red-excitable SYTO probes

Donald Wlodkowic, Joanna Skommer, Chris Hillier, Zbigniew Darzynkiewicz

Research output: Contribution to journalArticle

Abstract

Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.

Original languageEnglish
Pages (from-to)563-569
Number of pages7
JournalCytometry: Part A
Volume73A
Issue number6
DOIs
Publication statusPublished - 1 Jun 2008

Keywords

  • apoptosis
  • flow cytometry
  • anticancer drugs

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    Wlodkowic, D., Skommer, J., Hillier, C., & Darzynkiewicz, Z. (2008). Multiparameter detection of apoptosis using red-excitable SYTO probes. Cytometry: Part A, 73A(6), 563-569. https://doi.org/10.1002/cyto.a.20564