Abstract
Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.
| Original language | English |
|---|---|
| Pages (from-to) | 563-569 |
| Number of pages | 7 |
| Journal | Cytometry: Part A |
| Volume | 73A |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Jun 2008 |
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Keywords
- apoptosis
- flow cytometry
- anticancer drugs
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Multiparameter detection of apoptosis using red-excitable SYTO probes. / Wlodkowic, Donald; Skommer, Joanna; Hillier, Chris; Darzynkiewicz, Zbigniew.
In: Cytometry: Part A, Vol. 73A, No. 6, 01.06.2008, p. 563-569.Research output: Contribution to journal › Article
TY - JOUR
T1 - Multiparameter detection of apoptosis using red-excitable SYTO probes
AU - Wlodkowic, Donald
AU - Skommer, Joanna
AU - Hillier, Chris
AU - Darzynkiewicz, Zbigniew
N1 - Originally published in: Cytometry: Part A (2008), 73A (6), pp.563-569.
PY - 2008/6/1
Y1 - 2008/6/1
N2 - Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.
AB - Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent SYTO probes, we provide herein further insights into applicability of novel red-excitable SYTO stains (SYTO 17, 59–64) for multiparameter analysis of cell fate. In particular, SYTO 62 appears to be a spectrally favorable candidate.
KW - apoptosis
KW - flow cytometry
KW - anticancer drugs
U2 - 10.1002/cyto.a.20564
DO - 10.1002/cyto.a.20564
M3 - Article
VL - 73A
SP - 563
EP - 569
JO - Cytometry: Part A
JF - Cytometry: Part A
SN - 1552-4922
IS - 6
ER -