@article{3615734504714118973031aa7c5dcc54,
title = "Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates",
abstract = "The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.",
keywords = "Biofilm, Fluorometry, Guidelines, Microplate, Reproducibility, Spectrophotometry",
author = "Jontana Allkja and Thomas Bjarnsholt and Tom Coenye and Paul Cos and Adyary Fallarero and Harrison, {Joe J.} and Lopes, {Susana P.} and Antonio Oliver and Pereira, {Maria Olivia} and Gordon Ramage and Shirtliff, {Mark E.} and Paul Stoodley and Webb, {Jeremy S.} and Zaat, {Sebastian A.J.} and Goeres, {Darla M.} and Azevedo, {Nuno Filipe}",
note = "Funding Information: This project has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sklodowska – Curie grant agreement No 722467 , as part of the Print-Aid consortium. The information and views set out in this article are those of the authors and do not necessarily reflect the official opinion of the European Union. Neither the European Union institutions and bodies nor any person acting on their behalf may be held responsible for the use which may be made of the information contained therein. Funding Information: We would like to thank Prof. Dr. Mark E. Shirtliff for his contribution in the development of this guideline and the review of early manuscript drafts prior to his untimely death. We would also like to thank Jill Story (Montana State University, Center for Biofilm Engineering) for designing Fig. 1. This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska ? Curie grant agreement No 722467, as part of the Print-Aid consortium. The information and views set out in this article are those of the authors and do not necessarily reflect the official opinion of the European Union. Neither the European Union institutions and bodies nor any person acting on their behalf may be held responsible for the use which may be made of the information contained therein. This work received additional financial support by: project UID/EQU/00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy ? LEPABE funded by national funds through FCT/MCTES (PIDDAC); Project ?LEPABE-2-ECO-INNOVATION? ? NORTE-01-0145-FEDER-000005, funded by Norte Portugal Regional Operational Programme (NORTE 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Funding Information: This work received additional financial support by: project UID/EQU/00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE funded by national funds through FCT / MCTES (PIDDAC); Project “LEPABE-2-ECO-INNOVATION” – NORTE-01-0145-FEDER-000005 , funded by Norte Portugal Regional Operational Programme ( NORTE 2020 ), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).",
year = "2020",
month = dec,
doi = "10.1016/j.bioflm.2019.100010",
language = "English",
volume = "2",
journal = "Biofilm",
issn = "2590-2075",
publisher = "Elsevier B.V.",
}