Abstract
| Original language | English |
|---|---|
| Journal | Ecotoxicology and Environmental Safety |
| Publication status | Accepted/In press - 16 Jan 2020 |
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Keywords
- pharmaceuticals
- anaerobic biodegradation
- LC-HRMS
- Aliivibrio fischeri
- Lactuca sativa
- Raphidocelis subcaptata
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Mechanistic and ecotoxicological studies of amoxicillin removal through anaerobic degradation systems. / Busto, Raquel Viera; Roberts, Joanne; Hunter, Colin; Escudero, Ania; Helwig, Karin; Coelho, Lucia Helena Gomes .
In: Ecotoxicology and Environmental Safety, 16.01.2020.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mechanistic and ecotoxicological studies of amoxicillin removal through anaerobic degradation systems
AU - Busto, Raquel Viera
AU - Roberts, Joanne
AU - Hunter, Colin
AU - Escudero, Ania
AU - Helwig, Karin
AU - Coelho, Lucia Helena Gomes
N1 - *1-1-50* Acceptance in SAN AAM: 12m embargo - NYP 21/1/20 DC
PY - 2020/1/16
Y1 - 2020/1/16
N2 - Many studies have been conducted on the evaluation and monitoring of micropollutants and by-products in wastewater treatment plants. Considering the increase in the production and consumption of emerging contaminants, such as drugs, personal care products, and plasticisers, it is necessary to conduct studies that support the elaboration of laws and regulations that promote the environmentally sustainable use of sludge and effluents. In this work, the biological degradation of amoxicillin was studied under two anaerobic conditions: i) using a 6 L reactor operated under semi-continuous flow; and ii) a batch system with 100 mL sealed glass syringes. According to the statistical analysis, amoxicillin was completelyremoved from the systems, but biogas production inhibition was observed (p < 0.05). Liquid chromatography-high-resolution mass spectrometry analysis identified amoxicillin penicilloic acid, amoxilloic acid, amoxicillin diketopiperazine and phenol hydroxypyrazine as by-products under anaerobic conditions. Ecotoxicity tests on effluent treated under the batch conditions showed that the addition of higher amounts of amoxicillin inhibited the target species Aliivibrio fischeri and Raphidocelis subcaptata, causing functional decreases of 28.5% and 22.2% when the antibiotic concentration was 2,500 μg L-1. A. fischeri was the most sensitive organism to effluent treated under semi-continuous flow conditions; a continuous reduction inbioluminescence of up to 88.8% was observed after 39 days of feeding, which was associated with by-products accumulation due to unbalanced conditions during anaerobic digestion. Changes in the physico-chemical characteristics of the effluent caused the accumulation and removal o 34 f AMX-DKP IV and modified the toxicity to Lactuca sativa and R. subcapitata.
AB - Many studies have been conducted on the evaluation and monitoring of micropollutants and by-products in wastewater treatment plants. Considering the increase in the production and consumption of emerging contaminants, such as drugs, personal care products, and plasticisers, it is necessary to conduct studies that support the elaboration of laws and regulations that promote the environmentally sustainable use of sludge and effluents. In this work, the biological degradation of amoxicillin was studied under two anaerobic conditions: i) using a 6 L reactor operated under semi-continuous flow; and ii) a batch system with 100 mL sealed glass syringes. According to the statistical analysis, amoxicillin was completelyremoved from the systems, but biogas production inhibition was observed (p < 0.05). Liquid chromatography-high-resolution mass spectrometry analysis identified amoxicillin penicilloic acid, amoxilloic acid, amoxicillin diketopiperazine and phenol hydroxypyrazine as by-products under anaerobic conditions. Ecotoxicity tests on effluent treated under the batch conditions showed that the addition of higher amounts of amoxicillin inhibited the target species Aliivibrio fischeri and Raphidocelis subcaptata, causing functional decreases of 28.5% and 22.2% when the antibiotic concentration was 2,500 μg L-1. A. fischeri was the most sensitive organism to effluent treated under semi-continuous flow conditions; a continuous reduction inbioluminescence of up to 88.8% was observed after 39 days of feeding, which was associated with by-products accumulation due to unbalanced conditions during anaerobic digestion. Changes in the physico-chemical characteristics of the effluent caused the accumulation and removal o 34 f AMX-DKP IV and modified the toxicity to Lactuca sativa and R. subcapitata.
KW - pharmaceuticals
KW - anaerobic biodegradation
KW - LC-HRMS
KW - Aliivibrio fischeri
KW - Lactuca sativa
KW - Raphidocelis subcaptata
M3 - Article
ER -