Mechanistic and ecotoxicological studies of amoxicillin removal through anaerobic degradation systems

Raquel Viera Busto*, Joanne Roberts, Colin Hunter, Ania Escudero, Karin Helwig, Lucia Helena Gomes Coelho

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Many studies have been conducted on the evaluation and monitoring of micropollutants and by-products in wastewater treatment plants. Considering the increase in the production and consumption of emerging contaminants, such as drugs, personal care products, and plasticisers, it is necessary to conduct studies that support the elaboration of laws and regulations that promote the environmentally sustainable use of sludge and effluents. In this work, the biological degradation of amoxicillin was studied under two anaerobic conditions: i) using a 6 L reactor operated under semi-continuous flow; and ii) a batch system with 100 mL sealed glass syringes. According to the statistical analysis, amoxicillin was completelyremoved from the systems, but biogas production inhibition was observed (p < 0.05). Liquid chromatography-high-resolution mass spectrometry analysis identified amoxicillin penicilloic acid, amoxilloic acid, amoxicillin diketopiperazine and phenol hydroxypyrazine as by-products under anaerobic conditions. Ecotoxicity tests on effluent treated under the batch conditions showed that the addition of higher amounts of amoxicillin inhibited the target species Aliivibrio fischeri and Raphidocelis subcaptata, causing functional decreases of 28.5% and 22.2% when the antibiotic concentration was 2,500 μg L-1. A. fischeri was the most sensitive organism to effluent treated under semi-continuous flow conditions; a continuous reduction inbioluminescence of up to 88.8% was observed after 39 days of feeding, which was associated with by-products accumulation due to unbalanced conditions during anaerobic digestion. Changes in the physico-chemical characteristics of the effluent caused the accumulation and removal o 34 f AMX-DKP IV and modified the toxicity to Lactuca sativa and R. subcapitata.
Original languageEnglish
JournalEcotoxicology and Environmental Safety
Publication statusAccepted/In press - 16 Jan 2020

Fingerprint

effluent
degradation
anoxic conditions
acid
biogas
antibiotics
liquid chromatography
phenol
statistical analysis
drug
mass spectrometry
glass
sludge
toxicity
removal
pollutant
monitoring
by-product
regulation
anaerobic digestion

Keywords

  • pharmaceuticals
  • anaerobic biodegradation
  • LC-HRMS
  • Aliivibrio fischeri
  • Lactuca sativa
  • Raphidocelis subcaptata

Cite this

@article{9e4da36121c04f48bc961418f9b48a36,
title = "Mechanistic and ecotoxicological studies of amoxicillin removal through anaerobic degradation systems",
abstract = "Many studies have been conducted on the evaluation and monitoring of micropollutants and by-products in wastewater treatment plants. Considering the increase in the production and consumption of emerging contaminants, such as drugs, personal care products, and plasticisers, it is necessary to conduct studies that support the elaboration of laws and regulations that promote the environmentally sustainable use of sludge and effluents. In this work, the biological degradation of amoxicillin was studied under two anaerobic conditions: i) using a 6 L reactor operated under semi-continuous flow; and ii) a batch system with 100 mL sealed glass syringes. According to the statistical analysis, amoxicillin was completelyremoved from the systems, but biogas production inhibition was observed (p < 0.05). Liquid chromatography-high-resolution mass spectrometry analysis identified amoxicillin penicilloic acid, amoxilloic acid, amoxicillin diketopiperazine and phenol hydroxypyrazine as by-products under anaerobic conditions. Ecotoxicity tests on effluent treated under the batch conditions showed that the addition of higher amounts of amoxicillin inhibited the target species Aliivibrio fischeri and Raphidocelis subcaptata, causing functional decreases of 28.5{\%} and 22.2{\%} when the antibiotic concentration was 2,500 μg L-1. A. fischeri was the most sensitive organism to effluent treated under semi-continuous flow conditions; a continuous reduction inbioluminescence of up to 88.8{\%} was observed after 39 days of feeding, which was associated with by-products accumulation due to unbalanced conditions during anaerobic digestion. Changes in the physico-chemical characteristics of the effluent caused the accumulation and removal o 34 f AMX-DKP IV and modified the toxicity to Lactuca sativa and R. subcapitata.",
keywords = "pharmaceuticals, anaerobic biodegradation, LC-HRMS, Aliivibrio fischeri, Lactuca sativa, Raphidocelis subcaptata",
author = "Busto, {Raquel Viera} and Joanne Roberts and Colin Hunter and Ania Escudero and Karin Helwig and Coelho, {Lucia Helena Gomes}",
note = "*1-1-50* Acceptance in SAN AAM: 12m embargo - NYP 21/1/20 DC",
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month = "1",
day = "16",
language = "English",

}

Mechanistic and ecotoxicological studies of amoxicillin removal through anaerobic degradation systems. / Busto, Raquel Viera; Roberts, Joanne; Hunter, Colin; Escudero, Ania; Helwig, Karin; Coelho, Lucia Helena Gomes .

In: Ecotoxicology and Environmental Safety, 16.01.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mechanistic and ecotoxicological studies of amoxicillin removal through anaerobic degradation systems

AU - Busto, Raquel Viera

AU - Roberts, Joanne

AU - Hunter, Colin

AU - Escudero, Ania

AU - Helwig, Karin

AU - Coelho, Lucia Helena Gomes

N1 - *1-1-50* Acceptance in SAN AAM: 12m embargo - NYP 21/1/20 DC

PY - 2020/1/16

Y1 - 2020/1/16

N2 - Many studies have been conducted on the evaluation and monitoring of micropollutants and by-products in wastewater treatment plants. Considering the increase in the production and consumption of emerging contaminants, such as drugs, personal care products, and plasticisers, it is necessary to conduct studies that support the elaboration of laws and regulations that promote the environmentally sustainable use of sludge and effluents. In this work, the biological degradation of amoxicillin was studied under two anaerobic conditions: i) using a 6 L reactor operated under semi-continuous flow; and ii) a batch system with 100 mL sealed glass syringes. According to the statistical analysis, amoxicillin was completelyremoved from the systems, but biogas production inhibition was observed (p < 0.05). Liquid chromatography-high-resolution mass spectrometry analysis identified amoxicillin penicilloic acid, amoxilloic acid, amoxicillin diketopiperazine and phenol hydroxypyrazine as by-products under anaerobic conditions. Ecotoxicity tests on effluent treated under the batch conditions showed that the addition of higher amounts of amoxicillin inhibited the target species Aliivibrio fischeri and Raphidocelis subcaptata, causing functional decreases of 28.5% and 22.2% when the antibiotic concentration was 2,500 μg L-1. A. fischeri was the most sensitive organism to effluent treated under semi-continuous flow conditions; a continuous reduction inbioluminescence of up to 88.8% was observed after 39 days of feeding, which was associated with by-products accumulation due to unbalanced conditions during anaerobic digestion. Changes in the physico-chemical characteristics of the effluent caused the accumulation and removal o 34 f AMX-DKP IV and modified the toxicity to Lactuca sativa and R. subcapitata.

AB - Many studies have been conducted on the evaluation and monitoring of micropollutants and by-products in wastewater treatment plants. Considering the increase in the production and consumption of emerging contaminants, such as drugs, personal care products, and plasticisers, it is necessary to conduct studies that support the elaboration of laws and regulations that promote the environmentally sustainable use of sludge and effluents. In this work, the biological degradation of amoxicillin was studied under two anaerobic conditions: i) using a 6 L reactor operated under semi-continuous flow; and ii) a batch system with 100 mL sealed glass syringes. According to the statistical analysis, amoxicillin was completelyremoved from the systems, but biogas production inhibition was observed (p < 0.05). Liquid chromatography-high-resolution mass spectrometry analysis identified amoxicillin penicilloic acid, amoxilloic acid, amoxicillin diketopiperazine and phenol hydroxypyrazine as by-products under anaerobic conditions. Ecotoxicity tests on effluent treated under the batch conditions showed that the addition of higher amounts of amoxicillin inhibited the target species Aliivibrio fischeri and Raphidocelis subcaptata, causing functional decreases of 28.5% and 22.2% when the antibiotic concentration was 2,500 μg L-1. A. fischeri was the most sensitive organism to effluent treated under semi-continuous flow conditions; a continuous reduction inbioluminescence of up to 88.8% was observed after 39 days of feeding, which was associated with by-products accumulation due to unbalanced conditions during anaerobic digestion. Changes in the physico-chemical characteristics of the effluent caused the accumulation and removal o 34 f AMX-DKP IV and modified the toxicity to Lactuca sativa and R. subcapitata.

KW - pharmaceuticals

KW - anaerobic biodegradation

KW - LC-HRMS

KW - Aliivibrio fischeri

KW - Lactuca sativa

KW - Raphidocelis subcaptata

M3 - Article

ER -