Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18

Henrik Chart; Janice Spencer; Henry R. Smith; Bernard Rowe

doi:10.1016/S0378-1097(97)00011-6

Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18

Henrik Chart^*, Janice Spencer, Henry R. Smith, Bernard Rowe

^*Corresponding author for this work

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Enteroaggregative strains of Escherichia coli, belonging to serotypesO44:H18 and O126:H27, were used to show that magnesium ions were essential for the adhesion of these enteroaggregative strains to HEp-2 cells. The removal ofMg2+ ions from culture media was correlated with the inability of strains to produce an outer membrane-associated protein of 18 kDa and a pellicle. It was concluded that magnesium ions were directly involved with the expression of an18 kDa outer membrane-associated protein by strains of E. coli O126:H27 andO44:H18, and that the outer membrane-associated protein was involved in bothHEp-2 adhesion and pellicle formation.

Original language	English
Pages (from-to)	49-52
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(97)00011-6
Publication status	Published - 1 Mar 1997

Keywords

Escherichia coli
enteroaggregative strain
magnesium
O126:H27
O44:H18
outer membrane protein

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Documents and Links

10.1016/S0378-1097(97)00011-6

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title = "Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18",

abstract = "Enteroaggregative strains of Escherichia coli, belonging to serotypesO44:H18 and O126:H27, were used to show that magnesium ions were essential for the adhesion of these enteroaggregative strains to HEp-2 cells. The removal ofMg2+ ions from culture media was correlated with the inability of strains to produce an outer membrane-associated protein of 18 kDa and a pellicle. It was concluded that magnesium ions were directly involved with the expression of an18 kDa outer membrane-associated protein by strains of E. coli O126:H27 andO44:H18, and that the outer membrane-associated protein was involved in bothHEp-2 adhesion and pellicle formation.",

keywords = "Escherichia coli, enteroaggregative strain, magnesium, O126:H27, O44:H18, outer membrane protein",

author = "Henrik Chart and Janice Spencer and Smith, {Henry R.} and Bernard Rowe",

year = "1997",

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doi = "10.1016/S0378-1097(97)00011-6",

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publisher = "Oxford University Press (OUP)",

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TY - JOUR

T1 - Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18

AU - Chart, Henrik

AU - Spencer, Janice

AU - Smith, Henry R.

AU - Rowe, Bernard

PY - 1997/3/1

Y1 - 1997/3/1

N2 - Enteroaggregative strains of Escherichia coli, belonging to serotypesO44:H18 and O126:H27, were used to show that magnesium ions were essential for the adhesion of these enteroaggregative strains to HEp-2 cells. The removal ofMg2+ ions from culture media was correlated with the inability of strains to produce an outer membrane-associated protein of 18 kDa and a pellicle. It was concluded that magnesium ions were directly involved with the expression of an18 kDa outer membrane-associated protein by strains of E. coli O126:H27 andO44:H18, and that the outer membrane-associated protein was involved in bothHEp-2 adhesion and pellicle formation.

AB - Enteroaggregative strains of Escherichia coli, belonging to serotypesO44:H18 and O126:H27, were used to show that magnesium ions were essential for the adhesion of these enteroaggregative strains to HEp-2 cells. The removal ofMg2+ ions from culture media was correlated with the inability of strains to produce an outer membrane-associated protein of 18 kDa and a pellicle. It was concluded that magnesium ions were directly involved with the expression of an18 kDa outer membrane-associated protein by strains of E. coli O126:H27 andO44:H18, and that the outer membrane-associated protein was involved in bothHEp-2 adhesion and pellicle formation.

KW - Escherichia coli

KW - enteroaggregative strain

KW - magnesium

KW - O126:H27

KW - O44:H18

KW - outer membrane protein

U2 - 10.1016/S0378-1097(97)00011-6

DO - 10.1016/S0378-1097(97)00011-6

M3 - Article

C2 - 9066109

AN - SCOPUS:0031105367

VL - 148

SP - 49

EP - 52

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -

Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18

Abstract

Keywords

ASJC Scopus subject areas

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