Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18

Henrik Chart*, Janice Spencer, Henry R. Smith, Bernard Rowe

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Enteroaggregative strains of Escherichia coli, belonging to serotypesO44:H18 and O126:H27, were used to show that magnesium ions were essential for the adhesion of these enteroaggregative strains to HEp-2 cells. The removal ofMg2+ ions from culture media was correlated with the inability of strains to produce an outer membrane-associated protein of 18 kDa and a pellicle. It was concluded that magnesium ions were directly involved with the expression of an18 kDa outer membrane-associated protein by strains of E. coli O126:H27 andO44:H18, and that the outer membrane-associated protein was involved in bothHEp-2 adhesion and pellicle formation.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalFEMS Microbiology Letters
Volume148
Issue number1
DOIs
Publication statusPublished - 1 Mar 1997

Keywords

  • Escherichia coli
  • enteroaggregative strain
  • magnesium
  • O126:H27
  • O44:H18
  • outer membrane protein

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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