Knockdown of the zebrafish ortholog of the retinitis pigmentosa 2 (RP2) gene results in retinal degeneration

Xinhua Shu, Zhiqiang Zeng, Philippe Gautier, Alan Lennon, Milica Gakovic, Michael E. Cheetham, E. Elizabeth Patton, Alan F. Wright

Research output: Contribution to journalArticle

Abstract

The authors investigated the expression and function of the zebrafish ortholog of the retinitis pigmentosa 2 (RP2) gene. Zebrafish RP2 (ZFRP2) cDNA was isolated from adult eye mRNA by reverse transcription–polymerase chain reaction (RT-PCR). Gene expression was examined by RT-PCR. The deduced peptide sequence was aligned with RP2 orthologues from different species. Translational suppression (knockdown) of zebrafish RP2 was carried out by antisense morpholino-injection. The phenotype of ZFRP2 knockdown morphants was characterized by immunohistology and histology. Human wild-type and mutant RP2 mRNAs were coinjected with ZFRP2 morpholinos to test whether human RP2 mRNA could rescue ZFRP2 knockdown phenotypes.

Original languageEnglish
Pages (from-to)2960-2966
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number6
DOIs
Publication statusPublished - 1 May 2011

Keywords

  • ophthalmology
  • vision sciences
  • molecular biology

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