Kinetics of vitellogenin protein and mRNA induction and depuration in fish following laboratory and environmental exposure to oestrogens

John A. Craft*, Margaret Brown, Kate Dempsey, Jill Francey, Mark F. Kirby, Alexander P. Scott, Ioanna Katsiadaki, Craig D. Robinson, Ian M. Davies, Philippe Bradac, Colin F. Moffat

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Plaice, flounder and sand goby were exposed to ethynylestradiol (EE2) for 21 days and then followed for up to 31 days after removal of the oestrogen. Plasma vitellogenin (VTG) and hepatic VTG mRNA were determined in groups of fish sampled during the induction and post-exposure phases. VTG mRNA increased slightly earlier than plasma protein, but both reached maxima by 21 days. In contrast, VTG mRNA decayed much more rapidly than protein after EE2 exposure was terminated (typical values t(1/2) mRNA 3 days, protein 15-30 days). Vitellogenin and VTG mRNA thus measure different temporal events and this is illustrated by field data of male flounder in which both parameters have been determined. Few fish show co-ordinate increased VTG mRNA and vitellogenin but rather more fish have increased vitellogenin. Low level increases of VTG mRNA (5 X) is observed in some fish without increased vitellogenin and this may represent polymorphic differences between individual fish. Crown
Original languageEnglish
Pages (from-to)419-423
Number of pages5
JournalMarine Environmental Research
Volume58
Issue number2-5
Early online date30 Apr 2004
DOIs
Publication statusPublished - Aug 2004

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science
  • Pollution

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