Isolation and Identification of Cellulose-Degradation Bacteria from Cattle Slurry

Rahma Mogbel S Alharbi, Ole Pahl, Xinhua Shu, Colin Hunter

Research output: Contribution to conferencePoster

Abstract

Cellulases are the third most widely used group of enzymes obtained from microbial sources. Despite the large number of microorganisms available to degrade cellulose, few bacterial sources can be produced in sufficient quantities to completely hydrolyze cellulose. The present study was undertaken to investigate and identify cellulolytic bacteria found in agriculture waste in a state of anaerobic digestion.
For the purposes of the study, bacterial isolates were obtained from three samples of cattle slurry: (1) fresh, prior to treatment; (2) after one month of anaerobic digestion; (3) after six months of anaerobic digestion. We employed the Sanger Sequencing Method of detection for plasmid isolation from bacterial isolates, to identify bacteria up to the genus level, using the 16 S rRNA PCR technique.
Of all the isolates, the majority of the bacteria identified belonged in order of prevalence to: (1) the genera Bacteroides, Clostridium, Enterobacter, Lactobacillus and Bifidobacterium; (2) the genera Bacteroides, Bifidobacterium, Klebsiella, Enterococcus and Staphylococcus; (3) the genera Clostridium, Lactobacillus, Bifidobacterium, Staphylococcus and Campylabacter.
The study showed the bacterial community’s composition differed across all three samples, and depended on the times at which the samples were taken prior or after treatment. Furthermore, future work is indicated to assess the anaerobic digestion potential of these bacteria isolates by measuring produced methane gas. This can be done after investigating and identifying bacterial isolates up to the species level, using Pyrosequencing.
Original languageEnglish
Publication statusPublished - 4 Sep 2016

Fingerprint

Cellulose
Bifidobacterium
Digestion
Bacteria
Bacteroides
Clostridium
Lactobacillus
Staphylococcus
Cellulases
Enterobacter
Klebsiella
Methane
Enterococcus
Agriculture
Plasmids
Gases
Polymerase Chain Reaction
Enzymes

Keywords

  • Anaerobic digestion, Bacteria, Cellulose-Degradation, Cattle Slurry, 16S r RNA

Cite this

@conference{3358cef1e78a4982ac0005afb02c9e5a,
title = "Isolation and Identification of Cellulose-Degradation Bacteria from Cattle Slurry",
abstract = "Cellulases are the third most widely used group of enzymes obtained from microbial sources. Despite the large number of microorganisms available to degrade cellulose, few bacterial sources can be produced in sufficient quantities to completely hydrolyze cellulose. The present study was undertaken to investigate and identify cellulolytic bacteria found in agriculture waste in a state of anaerobic digestion. For the purposes of the study, bacterial isolates were obtained from three samples of cattle slurry: (1) fresh, prior to treatment; (2) after one month of anaerobic digestion; (3) after six months of anaerobic digestion. We employed the Sanger Sequencing Method of detection for plasmid isolation from bacterial isolates, to identify bacteria up to the genus level, using the 16 S rRNA PCR technique.Of all the isolates, the majority of the bacteria identified belonged in order of prevalence to: (1) the genera Bacteroides, Clostridium, Enterobacter, Lactobacillus and Bifidobacterium; (2) the genera Bacteroides, Bifidobacterium, Klebsiella, Enterococcus and Staphylococcus; (3) the genera Clostridium, Lactobacillus, Bifidobacterium, Staphylococcus and Campylabacter. The study showed the bacterial community’s composition differed across all three samples, and depended on the times at which the samples were taken prior or after treatment. Furthermore, future work is indicated to assess the anaerobic digestion potential of these bacteria isolates by measuring produced methane gas. This can be done after investigating and identifying bacterial isolates up to the species level, using Pyrosequencing.",
keywords = "Anaerobic digestion, Bacteria, Cellulose-Degradation, Cattle Slurry, 16S r RNA",
author = "Alharbi, {Rahma Mogbel S} and Ole Pahl and Xinhua Shu and Colin Hunter",
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}

Isolation and Identification of Cellulose-Degradation Bacteria from Cattle Slurry. / Alharbi, Rahma Mogbel S; Pahl, Ole; Shu, Xinhua; Hunter, Colin.

2016.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Isolation and Identification of Cellulose-Degradation Bacteria from Cattle Slurry

AU - Alharbi, Rahma Mogbel S

AU - Pahl, Ole

AU - Shu, Xinhua

AU - Hunter, Colin

N1 - Acceptance email req'd 260718 DC. Clarification sought re: template 070818 DC. Confirmed as poster, so template updated and acceptance email no longer required 090818 DC.

PY - 2016/9/4

Y1 - 2016/9/4

N2 - Cellulases are the third most widely used group of enzymes obtained from microbial sources. Despite the large number of microorganisms available to degrade cellulose, few bacterial sources can be produced in sufficient quantities to completely hydrolyze cellulose. The present study was undertaken to investigate and identify cellulolytic bacteria found in agriculture waste in a state of anaerobic digestion. For the purposes of the study, bacterial isolates were obtained from three samples of cattle slurry: (1) fresh, prior to treatment; (2) after one month of anaerobic digestion; (3) after six months of anaerobic digestion. We employed the Sanger Sequencing Method of detection for plasmid isolation from bacterial isolates, to identify bacteria up to the genus level, using the 16 S rRNA PCR technique.Of all the isolates, the majority of the bacteria identified belonged in order of prevalence to: (1) the genera Bacteroides, Clostridium, Enterobacter, Lactobacillus and Bifidobacterium; (2) the genera Bacteroides, Bifidobacterium, Klebsiella, Enterococcus and Staphylococcus; (3) the genera Clostridium, Lactobacillus, Bifidobacterium, Staphylococcus and Campylabacter. The study showed the bacterial community’s composition differed across all three samples, and depended on the times at which the samples were taken prior or after treatment. Furthermore, future work is indicated to assess the anaerobic digestion potential of these bacteria isolates by measuring produced methane gas. This can be done after investigating and identifying bacterial isolates up to the species level, using Pyrosequencing.

AB - Cellulases are the third most widely used group of enzymes obtained from microbial sources. Despite the large number of microorganisms available to degrade cellulose, few bacterial sources can be produced in sufficient quantities to completely hydrolyze cellulose. The present study was undertaken to investigate and identify cellulolytic bacteria found in agriculture waste in a state of anaerobic digestion. For the purposes of the study, bacterial isolates were obtained from three samples of cattle slurry: (1) fresh, prior to treatment; (2) after one month of anaerobic digestion; (3) after six months of anaerobic digestion. We employed the Sanger Sequencing Method of detection for plasmid isolation from bacterial isolates, to identify bacteria up to the genus level, using the 16 S rRNA PCR technique.Of all the isolates, the majority of the bacteria identified belonged in order of prevalence to: (1) the genera Bacteroides, Clostridium, Enterobacter, Lactobacillus and Bifidobacterium; (2) the genera Bacteroides, Bifidobacterium, Klebsiella, Enterococcus and Staphylococcus; (3) the genera Clostridium, Lactobacillus, Bifidobacterium, Staphylococcus and Campylabacter. The study showed the bacterial community’s composition differed across all three samples, and depended on the times at which the samples were taken prior or after treatment. Furthermore, future work is indicated to assess the anaerobic digestion potential of these bacteria isolates by measuring produced methane gas. This can be done after investigating and identifying bacterial isolates up to the species level, using Pyrosequencing.

KW - Anaerobic digestion, Bacteria, Cellulose-Degradation, Cattle Slurry, 16S r RNA

M3 - Poster

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