Investigating dual-species candida auris and staphylococcal biofilm antiseptic challenge.

Dolunay Gülmez; Jason L. Brown; Mark C. Butcher; Christopher Delaney; Ryan Kean; Gordon Ramage; Bryn Short

doi:10.3390/antibiotics11070931

Investigating dual-species candida auris and staphylococcal biofilm antiseptic challenge.

Dolunay Gülmez^*, Jason L. Brown, Mark C. Butcher, Christopher Delaney, Ryan Kean, Gordon Ramage, Bryn Short^*

^*Corresponding author for this work

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

99 Downloads (Pure)

Abstract

Candida auris can persistently colonize human skin, alongside a diverse bacterial microbiome. In this study we aimed to investigate the efficacy of antiseptic activities on dual-species interkingdom biofilms containing staphylococci to determine if antiseptic tolerance was negatively impacted by dual-species biofilms. Chlorhexidine, povidone iodine, and hydrogen peroxide (H₂O₂), were able to significantly reduce biofilm viable cell counts following exposure at 2%, 10%, and 3%, respectively. Notably, H₂O₂-treated biofilms were able to significantly recover and considerably repopulate following treatment. Fortunately, inter-kingdom interactions in dual-species biofilms of C. auris and staphylococci did not increase the tolerance of C. auris against antiseptics in vitro. These data indicate mixed infections are manageable with chlorhexidine and povidone iodine, but caution should be exercised in the consideration of H₂O₂.

Original language	English
Article number	931
Journal	Antibiotics
Volume	11
Issue number	7
Early online date	11 Jul 2022
DOIs	https://doi.org/10.3390/antibiotics11070931
Publication status	Published - Jul 2022

Keywords

biofilm
polymicrobial
candida auris
antiseptic
staphylococcus spp

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Documents and Links

10.3390/antibiotics11070931

Gulmez, D. et al (2022) Investigating dual-species candida auris and staphylococcal biofilm antiseptic challenge
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) l
Final published version, 2.06 MBLicence: CC BY

Cite this

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title = "Investigating dual-species candida auris and staphylococcal biofilm antiseptic challenge.",

abstract = "Candida auris can persistently colonize human skin, alongside a diverse bacterial microbiome. In this study we aimed to investigate the efficacy of antiseptic activities on dual-species interkingdom biofilms containing staphylococci to determine if antiseptic tolerance was negatively impacted by dual-species biofilms. Chlorhexidine, povidone iodine, and hydrogen peroxide (H2O2), were able to significantly reduce biofilm viable cell counts following exposure at 2%, 10%, and 3%, respectively. Notably, H2O2-treated biofilms were able to significantly recover and considerably repopulate following treatment. Fortunately, inter-kingdom interactions in dual-species biofilms of C. auris and staphylococci did not increase the tolerance of C. auris against antiseptics in vitro. These data indicate mixed infections are manageable with chlorhexidine and povidone iodine, but caution should be exercised in the consideration of H2O2.",

keywords = "biofilm, polymicrobial, candida auris, antiseptic, staphylococcus spp",

author = "Dolunay G{\"u}lmez and Brown, {Jason L.} and Butcher, {Mark C.} and Christopher Delaney and Ryan Kean and Gordon Ramage and Bryn Short",

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T1 - Investigating dual-species candida auris and staphylococcal biofilm antiseptic challenge.

AU - Gülmez, Dolunay

AU - Brown, Jason L.

AU - Butcher, Mark C.

AU - Delaney, Christopher

AU - Kean, Ryan

AU - Ramage, Gordon

AU - Short, Bryn

PY - 2022/7

Y1 - 2022/7

N2 - Candida auris can persistently colonize human skin, alongside a diverse bacterial microbiome. In this study we aimed to investigate the efficacy of antiseptic activities on dual-species interkingdom biofilms containing staphylococci to determine if antiseptic tolerance was negatively impacted by dual-species biofilms. Chlorhexidine, povidone iodine, and hydrogen peroxide (H2O2), were able to significantly reduce biofilm viable cell counts following exposure at 2%, 10%, and 3%, respectively. Notably, H2O2-treated biofilms were able to significantly recover and considerably repopulate following treatment. Fortunately, inter-kingdom interactions in dual-species biofilms of C. auris and staphylococci did not increase the tolerance of C. auris against antiseptics in vitro. These data indicate mixed infections are manageable with chlorhexidine and povidone iodine, but caution should be exercised in the consideration of H2O2.

AB - Candida auris can persistently colonize human skin, alongside a diverse bacterial microbiome. In this study we aimed to investigate the efficacy of antiseptic activities on dual-species interkingdom biofilms containing staphylococci to determine if antiseptic tolerance was negatively impacted by dual-species biofilms. Chlorhexidine, povidone iodine, and hydrogen peroxide (H2O2), were able to significantly reduce biofilm viable cell counts following exposure at 2%, 10%, and 3%, respectively. Notably, H2O2-treated biofilms were able to significantly recover and considerably repopulate following treatment. Fortunately, inter-kingdom interactions in dual-species biofilms of C. auris and staphylococci did not increase the tolerance of C. auris against antiseptics in vitro. These data indicate mixed infections are manageable with chlorhexidine and povidone iodine, but caution should be exercised in the consideration of H2O2.

KW - biofilm

KW - polymicrobial

KW - candida auris

KW - antiseptic

KW - staphylococcus spp

U2 - 10.3390/antibiotics11070931

DO - 10.3390/antibiotics11070931

M3 - Article

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JO - Antibiotics

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M1 - 931

ER -

Investigating dual-species candida auris and staphylococcal biofilm antiseptic challenge.

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Documents and Links

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