Infection of hamsters with the UK Clostridium difficile ribotype 027 outbreak strain R20291

Anthony M. Buckley, Janice Spencer, Denise Candlish, June J. Irvine, Gillian R. Douce*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Clostridium difficile is the main cause of antibiotic-associated disease, a disease of high socio-economical importance that has recently been compounded by the global spread of the 027 (BI/NAP1/027) ribotype. Cdifficile cases attributed to ribotype 027 strains have high recurrence rates (up to 36 %) and increased disease severity. The hamster model of infection is widely accepted as an appropriate model for studying aspects of Cdifficile host–pathogen interactions. Using this model we characterized the infection kinetics of the UK 2006 outbreak strain, R20291.Hamsters were orally given a dose of clindamycin, followed 5 days later with10 000 Cdifficile spores. All 100 % of the hamsters succumbed to infection with a mean time to the clinical end point of 46.7 h. Colonization of the caecum and colon were observed 12 h post-infection reaching a maximum of approximately 3×104 c.f.u. per organ, but spores were not detected until 24 h post-infection. At 36 h post-infection Cdifficile numbers increased significantly to approximately 6×107 c.f.u. per organ where numbers remained high until the clinical end point. Increasing levels of in vivo toxin production coincided with increases in Cdifficile numbers in organs reaching a maximum at 36 h post-infection in the caecum. Epithelial destruction and polymorphonuclear leukocyte (PMN) recruitment occurred early on during infection (24 h)accumulating as gross microvilli damage, luminal PMN influx, and blood associated with mucosal muscle and microvilli. These data describe the fatal infection kinetics of the clinical UK epidemic Cdifficile strain R20291 in the hamster infection model.

Original languageEnglish
Pages (from-to)1174-1180
Number of pages7
JournalJournal of Medical Microbiology
Volume60
Issue number8
DOIs
Publication statusPublished - 1 Aug 2011

Keywords

  • hamsters
  • Clostridium difficile
  • antibiotic-associated disease

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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