Increased central and peripheral inflammation and inflammatory hyperalgesia in Zucker rat model of leptin receptor deficiency and genetic obesity

Tommaso Iannitti, Ann Graham, Sharron Dolan

Research output: Contribution to journalArticle

Abstract

This study investigated whether sensitivity to nociceptive stimuli is altered in obese rats using established models of inflammatory pain, and using real-time PCR, profiled alterations in expression of key adipokine and inflammatory mediator mRNA (adiponectin, tumor necrosis factor-a, interleukin-1ß, cyclooxygenase-2, inducible nitric oxide synthase (iNOS)) in spinal cord with obesity. Responses to thermal and mechanical stimulation of the hindpaw and paw oedema were assessed in adult male Zucker fatty rats (fa/fa) and their lean littermates (fa/–; n = 6–9 per group) in the absence of inflammation (acute nociception), then in response to intradermal hindpaw injection of carrageenan (3%; 50 µl) or capsaicin (10 µg; 50 µl) or hindpaw incision.
Original languageEnglish
Pages (from-to)1236-1245
Number of pages10
JournalExperimental Physiology
Volume97
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Zucker Rats
Leptin Receptors
Intradermal Injections
Adipokines
Nociception
Carrageenan
Capsaicin
Adiponectin
Hyperalgesia
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Interleukin-1
Real-Time Polymerase Chain Reaction
Edema
Spinal Cord
Tumor Necrosis Factor-alpha
Obesity
Hot Temperature
Inflammation
Pain

Keywords

  • inflammation
  • inflammatory hyperalgesia
  • genetic obesity

Cite this

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