Incorporation of connexins into plasma membranes and gap junctions

Patricia E.M. Martin*, W. Howard Evans

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

106 Citations (Scopus)

Abstract

Gap junctions are polymeric assemblies of aligned pairs of interacting hexameric connexon hemichannel units facilitating direct intercellular communication. The principal process leading to assembly of gap junctions involves the cotranslational insertion of connexin (Cx) proteins into the endoplasmic reticulum, followed by their rapid oligomeric association into homo- or heteromeric connexons that are trafficked via the Golgi apparatus to the plasma membrane. Oligomerisation is a high-fidelity process that determines connexon channel stoichiometry and conductance characteristics. A large number of mutations in Cx26 and Cx32 detected in genetic diseases have emphasised the requirement for precise oligomerisation of connexins into hexameric connexons that traffic to the plasma membrane. Mutations in Cx43 are rare, and in the cardiovascular system, where it is the dominant connexin, disease changes are linked to its abundance and to gap junction remodelling. Connexins with short carboxyl tails may also be post-translationally inserted as oligomeric channels directly into plasma membranes. This mechanism of channel assembly is highly dependent on microtubule integrity and may allow cells to rapidly modulate gap junctional cross talk.
Original languageEnglish
Pages (from-to)378-387
Number of pages10
JournalCardiovascular Research
Volume62
Issue number2
DOIs
Publication statusPublished - May 2004
Externally publishedYes

Keywords

  • Connexin chimeric proteins
  • Connexins
  • Trafficking pathways

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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