In-vivo confocal microscopy of the corneal endothelium: comparison of three morphometry methods after corneal transplantation

Sven Jonuscheit; Michael Doughty; Kanna Ramaesh

doi:10.1038/eye.2011.121

In-vivo confocal microscopy of the corneal endothelium: comparison of three morphometry methods after corneal transplantation

Sven Jonuscheit, Michael Doughty, Kanna Ramaesh

Vision Sciences

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The purpose of this study was to assess the endothelium of corneal grafts by in vivo confocal microscopy (IVCM), and to evaluate an automated endothelial software system in comparison with a manual cell count and planimetry. Overall, 40 corneal grafts (20 deep anterior lamellar keratoplasties (DALKs) and 20 penetrating keratoplasties (PKs)) were assessed by scanning-slit IVCM. The endothelial cell density (ECD) was estimated with the automated and the manual cell count method of the instrument's Nidek Advanced Vision Information System (NAVIS) software. The results were compared with planimetry as the reference method, and the agreement was assessed.

Original language	English
Pages (from-to)	1130-1137
Number of pages	8
Journal	Eye
Volume	25
Issue number	9
DOIs	https://doi.org/10.1038/eye.2011.121
Publication status	Published - 1 Sept 2011

Keywords

corneal transplants
corneal endothelium
vision sciences
confocal microscopy

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10.1038/eye.2011.121

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title = "In-vivo confocal microscopy of the corneal endothelium: comparison of three morphometry methods after corneal transplantation",

abstract = "The purpose of this study was to assess the endothelium of corneal grafts by in vivo confocal microscopy (IVCM), and to evaluate an automated endothelial software system in comparison with a manual cell count and planimetry. Overall, 40 corneal grafts (20 deep anterior lamellar keratoplasties (DALKs) and 20 penetrating keratoplasties (PKs)) were assessed by scanning-slit IVCM. The endothelial cell density (ECD) was estimated with the automated and the manual cell count method of the instrument's Nidek Advanced Vision Information System (NAVIS) software. The results were compared with planimetry as the reference method, and the agreement was assessed.",

keywords = "corneal transplants, corneal endothelium, vision sciences, confocal microscopy",

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AB - The purpose of this study was to assess the endothelium of corneal grafts by in vivo confocal microscopy (IVCM), and to evaluate an automated endothelial software system in comparison with a manual cell count and planimetry. Overall, 40 corneal grafts (20 deep anterior lamellar keratoplasties (DALKs) and 20 penetrating keratoplasties (PKs)) were assessed by scanning-slit IVCM. The endothelial cell density (ECD) was estimated with the automated and the manual cell count method of the instrument's Nidek Advanced Vision Information System (NAVIS) software. The results were compared with planimetry as the reference method, and the agreement was assessed.

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In-vivo confocal microscopy of the corneal endothelium: comparison of three morphometry methods after corneal transplantation

Abstract

Keywords

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