Implications of antimicrobial combinations in complex wound biofilms containing fungi

Eleanor M. Townsend; Leighann Sherry; Ryan Kean; Donald Hansom; William G. Mackay; Craig Williams; John Butcher; Gordon Ramage

doi:10.1128/AAC.00672-17

Implications of antimicrobial combinations in complex wound biofilms containing fungi

Eleanor M. Townsend
, Leighann Sherry
, Ryan Kean
, Donald Hansom
, William G. Mackay
, Craig Williams
, John Butcher
, Gordon Ramage

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

198 Downloads (Pure)

Abstract

Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this, we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while monotreatment approaches influenced biofilm composition, this had no discernible effect on overall quantity. Only by combining bacterium- and fungus-specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.

Original language	English
Pages (from-to)	e00672
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	9
Early online date	10 Jul 2017
DOIs	https://doi.org/10.1128/AAC.00672-17
Publication status	Published - Sept 2017
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

antimicrobial agents
wound healing
biofilms
foot ulcers
Antimicrobial
Chronic wound
Candida
Biofilm
Polymicrobial
Biofilms/drug effects
Diabetic Foot/drug therapy
Humans
Candida albicans/drug effects
Ciprofloxacin/therapeutic use
Microbial Sensitivity Tests
Floxacillin/therapeutic use
Staphylococcus aureus/drug effects
Fluconazole/therapeutic use
Antifungal Agents/therapeutic use
Anti-Bacterial Agents/therapeutic use
Foot Ulcer/drug therapy
Pseudomonas aeruginosa/drug effects

ASJC Scopus subject areas

Pharmacology (medical)
Infectious Diseases
Pharmacology

Documents and Links

10.1128/AAC.00672-17

Townsend, E.M. et al (2017) Antimicrobial Agents and Chemotherapy article
This is the final author version of the article Townsend EM, Sherry L, Kean R, Hansom D, Mackay WG, Williams C, Butcher J, Ramage G. 2017. Implications of antimicrobial combinations in complex wound biofilms containing fungi. Antimicrob Agents Chemother 61:e00672-17. https://doi.org/10.1128/AAC.00672-17. Published by American Society for Microbiology. Copyright © 2017 American Society for Microbiology. All Rights Reserved.
Author accepted manuscript, 653 KB

Cite this

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title = "Implications of antimicrobial combinations in complex wound biofilms containing fungi",

abstract = "Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this, we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while monotreatment approaches influenced biofilm composition, this had no discernible effect on overall quantity. Only by combining bacterium- and fungus-specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.",

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AU - Townsend, Eleanor M.

AU - Sherry, Leighann

AU - Kean, Ryan

AU - Hansom, Donald

AU - Mackay, William G.

AU - Williams, Craig

AU - Butcher, John

AU - Ramage, Gordon

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AB - Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this, we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while monotreatment approaches influenced biofilm composition, this had no discernible effect on overall quantity. Only by combining bacterium- and fungus-specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.

KW - antimicrobial agents

KW - wound healing

KW - biofilms

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KW - Candida

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KW - Candida albicans/drug effects

KW - Ciprofloxacin/therapeutic use

KW - Microbial Sensitivity Tests

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KW - Staphylococcus aureus/drug effects

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KW - Antifungal Agents/therapeutic use

KW - Anti-Bacterial Agents/therapeutic use

KW - Foot Ulcer/drug therapy

KW - Pseudomonas aeruginosa/drug effects

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DO - 10.1128/AAC.00672-17

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SP - e00672

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 9

ER -

Implications of antimicrobial combinations in complex wound biofilms containing fungi

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Documents and Links

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Cite this