Image formation in structured illumination wide-field fluorescence microscopy

Dejan Karadaglic, Tony Wilson

    Research output: Contribution to journalArticle

    Abstract

    We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. The theoretical considerations are compared with and confirmed by experimental results.
    Original languageEnglish
    Pages (from-to)808-818
    Number of pages11
    JournalMicron
    Volume39
    Issue number7
    DOIs
    Publication statusPublished - Oct 2008

    Keywords

    • structured illumination
    • optical sectioning
    • optical microscopy
    • fluorescence microscopy
    • wide-field microscopy
    • confocal microscopy

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