Image formation in structured illumination wide-field fluorescence microscopy

Dejan Karadaglic; Tony Wilson

doi:10.1016/j.micron.2008.01.017

Image formation in structured illumination wide-field fluorescence microscopy

Research output: Contribution to journal › Article

Abstract

We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. The theoretical considerations are compared with and confirmed by experimental results.

Original language	English
Pages (from-to)	808-818
Number of pages	11
Journal	Micron
Volume	39
Issue number	7
DOIs	https://doi.org/10.1016/j.micron.2008.01.017
Publication status	Published - Oct 2008

Keywords

structured illumination
optical sectioning
optical microscopy
fluorescence microscopy
wide-field microscopy
confocal microscopy

Documents and Links

10.1016/j.micron.2008.01.017

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title = "Image formation in structured illumination wide-field fluorescence microscopy",

abstract = "We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. The theoretical considerations are compared with and confirmed by experimental results.",

keywords = "structured illumination, optical sectioning, optical microscopy, fluorescence microscopy, wide-field microscopy, confocal microscopy",

author = "Dejan Karadaglic and Tony Wilson",

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AU - Wilson, Tony

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AB - We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. The theoretical considerations are compared with and confirmed by experimental results.

KW - structured illumination

KW - optical sectioning

KW - optical microscopy

KW - fluorescence microscopy

KW - wide-field microscopy

KW - confocal microscopy

U2 - 10.1016/j.micron.2008.01.017

DO - 10.1016/j.micron.2008.01.017

M3 - Article

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EP - 818

JO - Micron

JF - Micron

SN - 0968-4328

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