Image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination

Dejan Karadaglic

doi:10.1016/j.micron.2007.10.001

Image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination

Dejan Karadaglic

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

We present a theoretical analysis of the image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination. We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. Some effects of spherical aberrations are included in the analysis. The theoretical considerations are compared with and confirmed by experimental results.

Original language	English
Pages (from-to)	302-310
Number of pages	9
Journal	Micron
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/j.micron.2007.10.001
Publication status	Published - Apr 2008

Keywords

structured illumination
optical sectioning
optical microscopy
brightfield microscopy
spherical aberrations

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Biomedical Engineering

Documents and Links

10.1016/j.micron.2007.10.001

Cite this

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title = "Image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination",

abstract = "We present a theoretical analysis of the image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination. We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. Some effects of spherical aberrations are included in the analysis. The theoretical considerations are compared with and confirmed by experimental results.",

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AU - Karadaglic, Dejan

PY - 2008/4

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N2 - We present a theoretical analysis of the image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination. We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. Some effects of spherical aberrations are included in the analysis. The theoretical considerations are compared with and confirmed by experimental results.

AB - We present a theoretical analysis of the image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination. We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. Some effects of spherical aberrations are included in the analysis. The theoretical considerations are compared with and confirmed by experimental results.

KW - structured illumination

KW - optical sectioning

KW - optical microscopy

KW - brightfield microscopy

KW - spherical aberrations

U2 - 10.1016/j.micron.2007.10.001

DO - 10.1016/j.micron.2007.10.001

M3 - Article

SN - 0968-4328

VL - 39

SP - 302

EP - 310

JO - Micron

JF - Micron

IS - 3

ER -

Image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination

Abstract

Keywords

ASJC Scopus subject areas

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