IL-33 exacerbates periodontal disease through induction of RANKL

J. Malcolm, R. A. Awang, J. Oliver-Bell, J.P. Butcher, L. Campbell, A. Adrados Planell, D. F. Lappin, S. Y. Fukada, C. J. Nile, F. Y. Liew, S. Culshaw

Research output: Contribution to journalArticle

Abstract

Cytokines mediate the balance between protective and destructive immunity in periodontitis. We sought to investigate the role of IL-33 in periodontitis. The expression of IL-33 in gingival tissue from healthy controls (n = 10) and patients with chronic periodontitis (n = 17) was investigated. Based on a murine model of periodontal disease, the function of IL-33 was determined first by administration of exogenous IL-33 and second by inhibition of IL-33 signaling using mice deficient in the IL-33 receptor ST2. Alveolar bone level, serum antibody, and lymphocyte responses were assessed in the murine model. Expression of IL-33 and ST2 was elevated in gingival tissues from patients with chronic periodontitis as compared with healthy tissues (P <0.05). Similarly, Il33 expression was higher in periodontal tissues of Porphyromonas gingivalis-infected mice as compared with sham-infected controls (P <0.05). IL-33 treatment of P. gingivalis-infected mice significantly exacerbated alveolar bone loss when compared with infection or IL-33 treatment alone (P <0.001). Conversely, P. gingivalis infection-induced alveolar bone loss was attenuated in mice lacking ST2. The percentages of T and B lymphocytes expressing nuclear factor kappa B ligand (RANKL) in the gingival tissues and T lymphocytes expressing RANKL in the cervical draining lymph nodes were higher in IL-33-treated P. gingivalis-infected mice versus phosphate buffered saline-treated P. gingivalis-infected controls (all P <0.001). Targeting the RANKL pathway by osteoprotegerin administration abrogated periodontal bone destruction in P. gingivalis-infected, IL-33-treated mice. These data demonstrate a previously unrecognized role for IL-33 in exacerbating bone loss in a RANKL-dependent manner in the context of bacterial infection and suggest that this pathway may be amenable to manipulation as a novel therapeutic target in periodontitis.
Original languageEnglish
Pages (from-to)968-975
Number of pages8
JournalJournal of Dental Research
Volume94
Issue number7
DOIs
Publication statusPublished - 1 Jul 2015

Keywords

  • ST2, porphyromonas gingivalis, cytokines, gingiva, periodontitis, OPG

Fingerprint Dive into the research topics of 'IL-33 exacerbates periodontal disease through induction of RANKL'. Together they form a unique fingerprint.

  • Cite this

    Malcolm, J., Awang, R. A., Oliver-Bell, J., Butcher, J. P., Campbell, L., Planell, A. A., Lappin, D. F., Fukada, S. Y., Nile, C. J., Liew, F. Y., & Culshaw, S. (2015). IL-33 exacerbates periodontal disease through induction of RANKL. Journal of Dental Research, 94(7), 968-975. https://doi.org/10.1177/0022034515577815