Identification of transcriptional effects of ethynyl oestradiol in male plaice (Pleuronectes platessa) by suppression subtractive hybridisation and a nylon macroarray

Margaret Brown*, Ian M. Davies, Colin F. Moffat, Craig Robinson, John Redshaw, John A. Craft

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially expressed in response to ethynyl oestradiol (EE2) exposure in liver from male plaice (Pleuronectes platessa) previously analysed for vitellogenin (VTG) induction. Characterisation of the cDNA clones identified many as VTG (2 genes) and zona radiata proteins (ZRP) (3 genes), but 40 encoded other proteins, with more than half cryptic. Further analysis identified 85 non-redundant clones suitable for array on nylon membrane. Radiolabelled cDNAs were prepared from hepatic mRNA from EE2 treated plaice (0 and 21 days) and hybridised with the arrayed clones. Analysis of the data showed that 11/17 novel, 21/22 VTG, 13/14 ZRP, 2/2 liver aspartic proteinase (LAP) and 8/10 other mRNAs were up-regulated by EE2 exposure.
Original languageEnglish
Pages (from-to)559-563
Number of pages5
JournalMarine Environmental Research
Volume58
Issue number2-5
Early online date30 Apr 2004
DOIs
Publication statusPublished - Aug 2004

Keywords

  • Endocrine disruption
  • Ethynyl oestradiol
  • Pleuronectes platessa
  • Suppression subtractive hybridisation
  • Transcription profiling

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science
  • Pollution

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