Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine

James C. Wood, Gary Quinn, Kristen M. Suling, Beth A. Oldmixon, Brian A. Van Tine, Robert Cina, Scott Arn, Christine A. Huang, Linda Scobie

Research output: Contribution to journalArticle

Abstract

The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swine peripheral blood mononuclear cells (PBMC) are recombinants of PERV-A with PERV-C. Here, we report that these recombinants are exogenous viruses in miniature swine; i.e., they are not present in the germ line DNA. These viruses were invariably present in miniature swine that transmitted PERV to human cells and were also identified in some miniature swine that lacked this ability. These data, together with the demonstration of the absence of both replication-competent PERV-A and recombinant PERV-A/C loci in the genome of miniature swine (L. Scobie, S. Taylor, J. C. Wood, K. M. Suling, G. Quinn, C. Patience, H.-J. Schuurman, and D. E. Onions, J. Virol. 78:2502-2509, 2004), indicate that exogenous PERV is the principal source of human-tropic virus in these animals. Interestingly, strong expression of PERV-C in PBMC correlated with an ability of the PBMC to transmit PERV-A/C recombinants in vitro, indicating that PERV-C may be an important factor affecting the production of human-tropic PERV. In light of these observations, the safety of clinical xenotransplantation from miniature swine will be most enhanced by the utilization of source animals that do not transmit PERV to either human or porcine cells. Such animals were identified within the miniature swine herd and may further enhance the safety of clinical xenotransplantation.

Original languageEnglish
JournalJournal of Virology
Publication statusPublished - 1 Mar 2004

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Retroviridae
Endogenous Retroviruses
Miniature Swine
miniature swine
tropics
Swine
swine
xenotransplantation
Heterologous Transplantation
mononuclear leukocytes
Blood Cells
Viruses
Safety
viruses
human cell lines
production economics
cells
Coculture Techniques
Germ Cells
germ cells

Keywords

  • xenotransplantation
  • porcine retroviruses
  • endogenous retroviruses

Cite this

Wood, J. C., Quinn, G., Suling, K. M., Oldmixon, B. A., Van Tine, B. A., Cina, R., ... Scobie, L. (2004). Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine. Journal of Virology.
Wood, James C. ; Quinn, Gary ; Suling, Kristen M. ; Oldmixon, Beth A. ; Van Tine, Brian A. ; Cina, Robert ; Arn, Scott ; Huang, Christine A. ; Scobie, Linda. / Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine. In: Journal of Virology. 2004.
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abstract = "The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swine peripheral blood mononuclear cells (PBMC) are recombinants of PERV-A with PERV-C. Here, we report that these recombinants are exogenous viruses in miniature swine; i.e., they are not present in the germ line DNA. These viruses were invariably present in miniature swine that transmitted PERV to human cells and were also identified in some miniature swine that lacked this ability. These data, together with the demonstration of the absence of both replication-competent PERV-A and recombinant PERV-A/C loci in the genome of miniature swine (L. Scobie, S. Taylor, J. C. Wood, K. M. Suling, G. Quinn, C. Patience, H.-J. Schuurman, and D. E. Onions, J. Virol. 78:2502-2509, 2004), indicate that exogenous PERV is the principal source of human-tropic virus in these animals. Interestingly, strong expression of PERV-C in PBMC correlated with an ability of the PBMC to transmit PERV-A/C recombinants in vitro, indicating that PERV-C may be an important factor affecting the production of human-tropic PERV. In light of these observations, the safety of clinical xenotransplantation from miniature swine will be most enhanced by the utilization of source animals that do not transmit PERV to either human or porcine cells. Such animals were identified within the miniature swine herd and may further enhance the safety of clinical xenotransplantation.",
keywords = "xenotransplantation, porcine retroviruses, endogenous retroviruses",
author = "Wood, {James C.} and Gary Quinn and Suling, {Kristen M.} and Oldmixon, {Beth A.} and {Van Tine}, {Brian A.} and Robert Cina and Scott Arn and Huang, {Christine A.} and Linda Scobie",
note = "Originally published in: Journal of Virology (2004), 78 (5), pp.2494-2501.",
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Wood, JC, Quinn, G, Suling, KM, Oldmixon, BA, Van Tine, BA, Cina, R, Arn, S, Huang, CA & Scobie, L 2004, 'Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine', Journal of Virology.

Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine. / Wood, James C.; Quinn, Gary; Suling, Kristen M.; Oldmixon, Beth A.; Van Tine, Brian A.; Cina, Robert; Arn, Scott; Huang, Christine A.; Scobie, Linda.

In: Journal of Virology, 01.03.2004.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine

AU - Wood, James C.

AU - Quinn, Gary

AU - Suling, Kristen M.

AU - Oldmixon, Beth A.

AU - Van Tine, Brian A.

AU - Cina, Robert

AU - Arn, Scott

AU - Huang, Christine A.

AU - Scobie, Linda

N1 - Originally published in: Journal of Virology (2004), 78 (5), pp.2494-2501.

PY - 2004/3/1

Y1 - 2004/3/1

N2 - The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swine peripheral blood mononuclear cells (PBMC) are recombinants of PERV-A with PERV-C. Here, we report that these recombinants are exogenous viruses in miniature swine; i.e., they are not present in the germ line DNA. These viruses were invariably present in miniature swine that transmitted PERV to human cells and were also identified in some miniature swine that lacked this ability. These data, together with the demonstration of the absence of both replication-competent PERV-A and recombinant PERV-A/C loci in the genome of miniature swine (L. Scobie, S. Taylor, J. C. Wood, K. M. Suling, G. Quinn, C. Patience, H.-J. Schuurman, and D. E. Onions, J. Virol. 78:2502-2509, 2004), indicate that exogenous PERV is the principal source of human-tropic virus in these animals. Interestingly, strong expression of PERV-C in PBMC correlated with an ability of the PBMC to transmit PERV-A/C recombinants in vitro, indicating that PERV-C may be an important factor affecting the production of human-tropic PERV. In light of these observations, the safety of clinical xenotransplantation from miniature swine will be most enhanced by the utilization of source animals that do not transmit PERV to either human or porcine cells. Such animals were identified within the miniature swine herd and may further enhance the safety of clinical xenotransplantation.

AB - The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swine peripheral blood mononuclear cells (PBMC) are recombinants of PERV-A with PERV-C. Here, we report that these recombinants are exogenous viruses in miniature swine; i.e., they are not present in the germ line DNA. These viruses were invariably present in miniature swine that transmitted PERV to human cells and were also identified in some miniature swine that lacked this ability. These data, together with the demonstration of the absence of both replication-competent PERV-A and recombinant PERV-A/C loci in the genome of miniature swine (L. Scobie, S. Taylor, J. C. Wood, K. M. Suling, G. Quinn, C. Patience, H.-J. Schuurman, and D. E. Onions, J. Virol. 78:2502-2509, 2004), indicate that exogenous PERV is the principal source of human-tropic virus in these animals. Interestingly, strong expression of PERV-C in PBMC correlated with an ability of the PBMC to transmit PERV-A/C recombinants in vitro, indicating that PERV-C may be an important factor affecting the production of human-tropic PERV. In light of these observations, the safety of clinical xenotransplantation from miniature swine will be most enhanced by the utilization of source animals that do not transmit PERV to either human or porcine cells. Such animals were identified within the miniature swine herd and may further enhance the safety of clinical xenotransplantation.

KW - xenotransplantation

KW - porcine retroviruses

KW - endogenous retroviruses

M3 - Article

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

ER -

Wood JC, Quinn G, Suling KM, Oldmixon BA, Van Tine BA, Cina R et al. Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine. Journal of Virology. 2004 Mar 1.