Identification of entero-aggregative Escherichia coli based on surface properties

H. Chart*, J. Spencer, H. R. Smith, B. Rowe

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Twenty-nine strains of Escherichia coli that adhere to HEp-2 cells with a ‘ ’ pattern (EAggEC), and four nonadherent control strains, were examined for the ability to hybridize with gene probes for aggregative (AA) and diffuse(DA)HEp-2 cell adhesion phenotypes. These strains were also tested for the ability to express an 18 kDa membrane-associated outer- membrane protein (MAP),to agglutinate erythrocytes, and to produce a pellicle during broth culture. Thirteen of the 29 HEp-2 adherent strains of E. coli hybridized with the gene probes for both AA and DA, and expressed an 18 kDa outer membrane protein (OMP) which was antigenically related to the MAP expressed by strains of E. coli O126:H27. The strains that did not carry the additional DA genes did not express an 18 kDa OMP. Although strains of EAggEC share the ability to adhere to HEp-2 cells with a stacked brick pattern, these strains exhibit a diverse range of physical and biochemical properties. From the results of this study, it was concluded that currently, the possession of EAggEC genes or the ability to adhere to HEp-2 cells in a stacked brick formation, remain the only reliable means of identifying EAggEC.

Original languageEnglish
Pages (from-to)712-717
Number of pages6
JournalJournal of Applied Microbiology
Volume83
Issue number6
DOIs
Publication statusPublished - Dec 1997

Keywords

  • Escherichia coli
  • EAggEC
  • genes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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