Identification of clinically relevant viridans group streptococci by phenotypic and genotypic analysis

C. Teles, A. Smith, Gordon Ramage, Sue Lang

Research output: Contribution to journalArticle

Abstract

Two phenotypic and three molecular methods were assessed for their ability to identify viridans group streptococci (VGS) to the species level. A panel of 23 clinical isolates, comprising strains isolated from infective endocarditis, blood cultures, pleural and peritoneal fluid, and 19 type/reference strains were analyzed. Identification was performed using two conventional phenotypic methods: API® rapid ID 32 Strep and the VITEK® 2 system, and genotypic analysis of the nucleotide sequence of the housekeeping gene sodA, restriction patterns generated by restriction fragment length polymorphism (RFLP) of the 16S rRNA gene and multilocus sequence analysis (MLSA) of seven housekeeping genes.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume30
Issue number2
DOIs
Publication statusPublished - Feb 2011

Keywords

  • viridans group streptococci
  • VGS

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