Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiolstar

Ioanna Katsiadaki, Timothy Williams, Jonathan Ball, Tim Bean, Matthew Sanders, Huifeng Wu, Eduarda Santos, Margaret Brown, Paul Baker, John A. Craft

Research output: Contribution to journalArticle

Abstract

An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1–100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls.

Original languageEnglish
Pages (from-to)174-187
Number of pages14
JournalAquatic Toxicology
Volume97
Issue number3
DOIs
Publication statusPublished - 1 May 2010

Keywords

  • choriogenins
  • vitellogenins
  • oestrogen
  • endocrine disruption
  • ecotoxicogenomics
  • aquatic toxicology

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    Katsiadaki, I., Williams, T., Ball, J., Bean, T., Sanders, M., Wu, H., Santos, E., Brown, M., Baker, P., & Craft, J. A. (2010). Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiolstar. Aquatic Toxicology, 97(3), 174-187. https://doi.org/10.1016/j.aquatox.2009.07.005