Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiolstar

Ioanna Katsiadaki; Timothy Williams; Jonathan Ball; Tim Bean; Matthew Sanders; Huifeng Wu; Eduarda Santos; Margaret Brown; Paul Baker; John A. Craft

doi:10.1016/j.aquatox.2009.07.005

Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiolstar

Ioanna Katsiadaki, Timothy Williams, Jonathan Ball, Tim Bean, Matthew Sanders, Huifeng Wu, Eduarda Santos, Margaret Brown, Paul Baker, John A. Craft

Biological and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1–100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls.

Original language	English
Pages (from-to)	174-187
Number of pages	14
Journal	Aquatic Toxicology
Volume	97
Issue number	3
DOIs	https://doi.org/10.1016/j.aquatox.2009.07.005
Publication status	Published - 1 May 2010

Keywords

choriogenins
vitellogenins
oestrogen
endocrine disruption
ecotoxicogenomics
aquatic toxicology

Documents and Links

10.1016/j.aquatox.2009.07.005

Cite this

@article{09ebb9fe75bf4fdcb4bdc87f1b27f84f,

title = "Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiolstar",

abstract = "An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1–100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls.",

keywords = "choriogenins, vitellogenins, oestrogen, endocrine disruption, ecotoxicogenomics, aquatic toxicology",

author = "Ioanna Katsiadaki and Timothy Williams and Jonathan Ball and Tim Bean and Matthew Sanders and Huifeng Wu and Eduarda Santos and Margaret Brown and Paul Baker and Craft, {John A.}",

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doi = "10.1016/j.aquatox.2009.07.005",

language = "English",

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journal = "Aquatic Toxicology",

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TY - JOUR

T1 - Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiolstar

AU - Katsiadaki, Ioanna

AU - Williams, Timothy

AU - Ball, Jonathan

AU - Bean, Tim

AU - Sanders, Matthew

AU - Wu, Huifeng

AU - Santos, Eduarda

AU - Brown, Margaret

AU - Baker, Paul

AU - Craft, John A.

N1 - <p>Originally published in: Aquatic Toxicology (2010), 97 (3), pp.174-187.</p>

PY - 2010/5/1

Y1 - 2010/5/1

N2 - An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1–100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls.

AB - An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1–100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls.

KW - choriogenins

KW - vitellogenins

KW - oestrogen

KW - endocrine disruption

KW - ecotoxicogenomics

KW - aquatic toxicology

U2 - 10.1016/j.aquatox.2009.07.005

DO - 10.1016/j.aquatox.2009.07.005

M3 - Article

VL - 97

SP - 174

EP - 187

JO - Aquatic Toxicology

JF - Aquatic Toxicology

IS - 3

ER -

Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiolstar

Abstract

Keywords

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